STUDIES ON TRANSFER RIBONUCLEIC-ACIDS AND RELATED COMPOUNDS .4. RIBOOLIGONUCLEOTIDE SYNTHESIS USING A PHOTOSENSITIVE O-NITROBENZYL PROTECTION AT 2'-HYDROXYL GROUP
STUDIES ON TRANSFER RIBONUCLEIC-ACIDS AND RELATED COMPOUNDS .4. RIBOOLIGONUCLEOTIDE SYNTHESIS USING A PHOTOSENSITIVE O-NITROBENZYL PROTECTION AT 2'-HYDROXYL GROUP
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DOI：
10.1093/nar/1.10.1351
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发表时间：
1974-01-01
影响因子：
14.9
通讯作者：
IKEHARA, M
中科院分区：
文献类型：
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作者：
OHTSUKA, E;TANAKA, S;IKEHARA, M
o-Nitrobenzyl group was introduced to the 2′-hydroxyl function of uridine via 2′, 3′-O-(dibutylstannylene) uridine. The benzylated uridine was protected at the 5′-hydroxyl group with monomethoxytrityl chloride and condensed with 2′,3′-O-dibenzoyluridine 5′-phosphate or N, N′,2′,3′-O-tetrabenzoyladenosine 5′-phosphate using dicyclohexylcarbodiimide (DCC). o-Nitrobenzyl ether linkage of the dinucleotides was removed by UV irradiation with wavelength longer than 320 nm. Deprotected UpU and UpA thus obtained were characterized by RNase A digestion.
