The origin of trypsin: Evidence for multiple gene duplications in trypsins
The origin of trypsin: Evidence for multiple gene duplications in trypsins
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DOI：
10.1007/pl00006393
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发表时间：
1998-09-01
影响因子：
3.9
通讯作者：
Petersen, SB
中科院分区：
文献类型：
--
作者：
Baptista, AM;Jonson, PH;Petersen, SB
丝氨酸蛋白酶中的胰酶家族是研究最多的蛋白质家族之一，在公共数据库中提供了丰富的氨基酸序列信息。由于类胰酶在自然界中广泛分布于生物体中，因此提出了可能的进化方案。提出了一种新的生物序列傅里叶变换方法(FOTOBIS)。该方法非常适合于识别蛋白质序列中短重复序列的大小和范围。本文用FOTOBIS对胰酶家族进行了分析，发现了串联基因复制的有力证据。目前发展胰酶的一条可能的进化路径涉及到一段15-18个核苷酸的小DNA片段的内在广泛串联基因复制，对应于五到六个氨基酸。这种祖传的胰酶基因随后被复制，导致了全尺寸胰酶的最早版本，当代胰酶就是从这个版本发展而来的。
The trypsin family of serine proteases is one of the most studied protein families, with a wealth of amino acid sequence information available in public databases. Since trypsin-like enzymes are widely distributed in living organisms in nature, likely evolutionary scenarios have been proposed. A novel methodology for Fourier transformation of biological sequences (FOTOBIS) is presented. The methodology is well suited for the identification of the size and extent of short repeats in protein sequences. In the present paper the trypsin family of enzymes is analyzed with FOTOBIS and strong evidence for tandem gene duplication is found. A likely evolutionary path for the development of present-day trypsins involved an intrinsic extensive tandem gene duplication of a small DNA fragment of 15-18 nucleotides, corresponding to five or six amino acids. This ancestral trypsin gene was subsequently duplicated, leading to the earliest version of a full-sized trypsin, from which the contemporary trypsins have developed.