Nutritionally induced body weight loss and ovarian quiescence in Shiba goats.
Nutritionally induced body weight loss and ovarian quiescence in Shiba goats.
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营养引起的柴山羊体重减轻和卵巢静止。
DOI：
10.1262/jrd.49.113
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发表时间：
2003
期刊：
影响因子：
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通讯作者：
Y. Kaneda
中科院分区：
文献类型：
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作者：
Tomomi Tanaka;Toshihiko Yamaguchi;H. Kamomae;Y. Kaneda
本试验选用4只雌性石坝山羊，研究了限饲减肥对发情周期的影响。在排卵后的第二天开始饮食限制。根据每周体重测量结果，以30%的能量需求量饲喂山羊干草块和稻草。每天通过经直肠超声检查监测卵巢，每天通过颈静脉穿刺采集血样用于卵巢类固醇分析。开始限食后，所有动物体重减轻并进入卵巢静止期。卵巢静止开始的间隔往往取决于开始限食时每只动物的体重。黄体中期孕酮的平均浓度卵巢静止期前末次发情周期（排卵后7 ~ 13天）的排卵量显著低于对照期正常发情周期（19.7 ± 2.8 vs 12.3 ± 2.2 ng/ml，P<0.05），而黄体期长度无显著差异，当黄体存在且孕酮浓度等于或大于1 ng/ml时，测定为15.8 +/- 1.5天vs 15.0 +/- 2.8天，P>0.1。在卵巢静止期开始时，随着黄体退化后孕酮水平的下降，卵泡期雌二醇浓度的升高和卵泡的生长往往受到抑制。目前的结果似乎与以前的研究结果一致，即营养诱导的体重减轻影响卵巢类固醇的分泌，并最终诱导卵巢静止。
Four female Shiba goats were used to determine the influence of body weight loss by dietary restriction on estrous cyclicity. The dietary restriction was started on the day following ovulation. The goats were fed hay cube and straw at an amount of 30% of energy requirement based on weekly body weight measurement. The ovaries were monitored daily by transrectal ultrasonography and blood samples were collected daily by jugular venipuncture for ovarian steroids analysis. After the start of food restriction, all animals lost body weight and entered ovarian quiescence. Intervals to the onset of ovarian quiescence tended to depend on the body weight of each animal at the start of food restriction. The mean concentration of progesterone during the mid-luteal phase (from 7 to 13 days after ovulation) in the last estrous cycle before ovarian quiescence was significantly lower than that in normal estrous cycle of the control period (19.7 +/- 2.8 vs 12.3 +/- 2.2 ng/ml, P<0.05), whereas there was no significant difference in the length of the luteal phase, determined as the period when corpora lutea existed and concentrations of progesterone were equal to or greater than 1 ng/ml (15.8 +/- 1.5 vs 15.0 +/- 2.8 days, P>0.1). A rise of estradiol concentration and follicular growth in the follicular phase following a decline of progesterone level after luteal regression tended to be suppressed at the onset of ovarian quiescence. It seems that the present results are consistent with previous findings that nutritionally induced body weight loss influences the secretion of ovarian steroids and eventually induces ovarian quiescence.