Acylsugars protect Nicotiana benthamiana against insect herbivory and desiccation
Acylsugars protect Nicotiana benthamiana against insect herbivory and desiccation
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DOI：
10.1007/s11103-021-01191-3
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发表时间：
2021-09-29
影响因子：
5.1
通讯作者：
Jander, Georg
中科院分区：
文献类型：
--
作者：
Feng, Honglin;Acosta-Gamboa, Lucia;Jander, Georg
烟草酰基糖酰基转移酶（ASAT）是防止干燥和昆虫植食所必需的。基因敲除突变为研究植物与蚜虫、烟粉虱的相互作用提供了新的资源。本氏烟草被广泛用作其他物种基因功能分析的瞬时表达平台。在毛状体中产生的酰基糖是在N.本萨米亚纳我们描述了N.本塞姆氏菌（benthamiana）的酰基糖谱，生物信息学鉴定了两个酰基糖酰基转移酶基因ASAT 1和ASAT 2，并使用CRISPR/Cas9诱变产生酰基糖缺陷型植物用于研究抗虫性和叶片失水。而asat 1突变减少积累，asat 2突变造成几乎完全耗尽叶酰基蔗糖。三个半翅目和三个鳞翅目草食动物存活，体重增加，和/或繁殖的asat 2突变体比野生型N。本萨米亚纳asat 1和asat 2突变都降低了叶片含水量，提高了叶片温度。我们的研究结果证实了两个ASAT蛋白在N.本塞姆氏菌酰基糖生物合成、抗虫性和脱水耐受性。蚜虫和粉虱在asat 2突变体上生长的改善将促进N.本塞姆那作为一个瞬时表达平台，用于昆虫效应基因和其他植物物种抗性基因的功能分析。类似地，asat 2突变体中酰基糖的缺失将使得能够通过瞬时表达分析来自其他茄科的酰基糖生物合成基因。
Key message Nicotiana benthamiana acylsugar acyltransferase (ASAT) is required for protection against desiccation and insect herbivory. Knockout mutations provide a new resource for investigation of plant-aphid and plant-whitefly interactions. Nicotiana benthamiana is used extensively as a transient expression platform for functional analysis of genes from other species. Acylsugars, which are produced in the trichomes, are a hypothesized cause of the relatively high insect resistance that is observed in N. benthamiana. We characterized the N. benthamiana acylsugar profile, bioinformatically identified two acylsugar acyltransferase genes, ASAT1 and ASAT2, and used CRISPR/Cas9 mutagenesis to produce acylsugar-deficient plants for investigation of insect resistance and foliar water loss. Whereas asat1 mutations reduced accumulation, asat2 mutations caused almost complete depletion of foliar acylsucroses. Three hemipteran and three lepidopteran herbivores survived, gained weight, and/or reproduced significantly better on asat2 mutants than on wildtype N. benthamiana. Both asat1 and asat2 mutations reduced the water content and increased leaf temperature. Our results demonstrate the specific function of two ASAT proteins in N. benthamiana acylsugar biosynthesis, insect resistance, and desiccation tolerance. The improved growth of aphids and whiteflies on asat2 mutants will facilitate the use of N. benthamiana as a transient expression platform for the functional analysis of insect effectors and resistance genes from other plant species. Similarly, the absence of acylsugars in asat2 mutants will enable analysis of acylsugar biosynthesis genes from other Solanaceae by transient expression.