CHARACTERIZATION AND SEQUENCE OF A MOUSE HSP70 GENE AND ITS EXPRESSION IN MOUSE-CELL LINES
CHARACTERIZATION AND SEQUENCE OF A MOUSE HSP70 GENE AND ITS EXPRESSION IN MOUSE-CELL LINES
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DOI：
10.1016/0378-1119(90)90302-8
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发表时间：
1990-03-15
期刊：
影响因子：
3.5
通讯作者：
CALDERWOOD, S
中科院分区：
文献类型：
--
作者：
HUNT, C;CALDERWOOD, S
小鼠热休克蛋白70基因家族的热诱导成员已被分离和表征。HSP 70基因1，在多聚腺苷酸化之前具有2.8kb的初级转录物，在多聚腺苷酸化之后具有3.1- 3.5kb的初级转录物。它包括一个645个核苷酸（nt）的3“-非翻译末端和一个225个核苷酸的5”前导序列。前导序列是不寻常的，因为它包含一个33-nt的三重串联重复元件接近ATG密码子。如预期的那样，一个单一的开放阅读框编码642个氨基酸（aa）的70-kDa的蛋白质与人hsp 70基因具有95%的aa同源性和91%的nt同源性。虽然通过5“-和3”-非翻译区没有同源性，但人和小鼠hsp 70基因启动子可以比对以得到65%的同源性。在研究的三种小鼠细胞系中，有两种在热休克期间表达3.1- 3.5 mRNA。一个细胞系还表达了一个额外的，较小的，2.6 kb的hsp 70 mRNA转录自一个单独的基因。小鼠红白血病细胞对热休克无反应，热休克既不诱导hsp 70 RNA。这些结果表明，细胞谱系对热休克的细胞反应有重大影响，并且这种反应并不普遍存在于所有细胞类型中。
A heat-inducible member of the mouse hsp70 gene family has been isolated and characterized. The gene, hsp70. 1, has a primary transcript of 2.8 kb before and 3.1-3.5 kb after polyadenylation. It includes a 645-nucleotide (nt) 3'' -nontranslated terminus and a 225-nt 5'' leader. The leader sequence is unusual in that it contains a 33-nt triple tandem repeat element proximal to the ATG codon. As expected, a single open reading frame encodes a 642-amino acid (aa) 70-kDa protein with 95% aa homology and 91% nt homology to a human hsp70 gene. While there is no homology through the 5''- and 3''-nontranslated regions, the human and mouse hsp70 gene promoters can be aligned to give 65% homology. Of three mouse cell lines studied, two expressed the 3.1- to 3.5 mRNA during heat shock. One line also expressed an additional, smaller, 2.6-kb hsp70 mRNA transcribed from a separate gene(s). Mouse erythroleukemia cells were unresponsive to heat shock, with neither hsp70 RNA being induced by heat shock. These results suggest that cell lineage has a major influence on the cellular response to heat shock and that the response is not universal in all cell types.