BDCA-1+, BDCA-2+ and BDCA-3+ dendritic cells in early human pregnancy decidua
BDCA-1+, BDCA-2+ and BDCA-3+ dendritic cells in early human pregnancy decidua
复制标题
人类妊娠早期蜕膜中的 BDCA-1、BDCA-2 和 BDCA-3 树突状细胞。
DOI：
10.1111/j.1365-2249.2007.03576.x
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发表时间：
2008-03-01
影响因子：
4.6
通讯作者：
Ma, Y. -Y.
中科院分区：
文献类型：
--
作者：
Ban, Y. -L.;Kong, B. -H.;Ma, Y. -Y.
树突状细胞（Dendritic cells，DCs）可以在不同的组织微环境中获得独特的特征或表型，并决定是否产生免疫或耐受。在蜕膜中观察到的DC与妊娠维持有关。然而，蜕膜DC亚群的精确分布及其表型特征在很大程度上是未知的。应用流式细胞术，我们在正常人早孕蜕膜中鉴定了三种DC亚群：BDCA-1（+）CD 19（-）CD 14（-）髓样DC 1型（MDC 1），BDCA-3（+）CD 14（-）髓样DC 2型（MDC 2）和BDCA-2（+）CD 123（+）浆细胞样DC（PDC）。蜕膜中MDC 1占单核细胞的百分比与外周血对照相似。蜕膜中MDC 2的百分比显著高于外周血对照，而PDC的百分比显著低于外周血对照。MDC 1和MDC 2亚群均表达低水平的人白细胞抗原D相关的CD 86和CD 80，表明未成熟髓样DC的特征。免疫球蛋白样转录本3，建议参与免疫耐受诱导，也表达在蜕膜MDC 1和MDC 2亚群。随着胎龄从6 ~ 9周增加，MDC 1的数量明显减少，MDC 2的数量明显增加。本研究首次证实了人蜕膜中存在三种以前未鉴定的BDCA-1（+）、BDCA-3（+）和BDCA-2（+）DC亚群，这些DC可能在维持妊娠中起重要作用。
Dendritic cells (DCs) can acquire unique features or phenotypes in different tissue microenvironments and decide whether immunity or tolerance develops. DCs observed within the decidua have been implicated in pregnancy maintenance. However, the precise distribution of decidual DC subsets and their phenotypic characteristics are largely unknown. Using flow cytometry, we identified three DC subsets in normal human first-trimester decidua: BDCA-1(+) CD19(-) CD14(-) myeloid DC type 1 (MDC1), BDCA-3(+) CD14(-) myeloid DC type 2 (MDC2) and BDCA-2(+) CD123(+) plasmacytoid DC (PDC). The percentage of MDC1 to mononuclear cells in the decidua was similar to that in the peripheral blood controls. The percentage of MDC2 in the decidua was significantly higher than that in the peripheral blood controls, whereas the percentage of PDC was significantly lower. Both MDC1 and MDC2 subsets expressed human leucocyte antigen D-related, CD86 and CD80 at low levels, suggesting a characteristic of immature myeloid DCs. Immunoglobulin-like transcript 3, suggested to be involved in immune tolerance induction, was also expressed on decidual MDC1 and MDC2 subsets. In addition, as gestational age increased from 6 to 9 weeks, the numbers of MDC1 decreased but MDC2 increased significantly. This is the first study to demonstrate the presence of three previously unidentified BDCA-1(+), BDCA-3(+) and BDCA-2(+) DC subsets in human decidua, these decidual DCs might play important role in the maintenance of pregnancy.