CD Measurements of β-Amyloid (1-40) and (1-42) in the Condensed Phase
CD Measurements of β-Amyloid (1-40) and (1-42) in the Condensed Phase
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DOI：
10.1002/bip.21543
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发表时间：
2011-02-01
期刊：
影响因子：
2.9
通讯作者：
Kuroda, Reiko
中科院分区：
文献类型：
--
作者：
Harada, Takunori;Kuroda, Reiko
薄膜中蛋白质/肽的圆二色性 (CD) 光谱可以提供有关聚集态结构的有价值的信息；然而，由于固相特有的宏观各向异性产生的伪影信号，很难定量估计二级结构含量。使用通用手性分光光度计 (UCS-1) 以及我们开发的测量和分析程序，我们可以获得与阿尔茨海默氏病相关的合成肽 Ab (1-40) 和 (1-42) 的铸型和 Langmuir-Blodgett (L-B) 膜的无伪影 CD 光谱。这项工作深入了解了结构转化和淀粉样蛋白样聚集的机制。 (C) 2010 年 Wiley 期刊公司。《生物聚合物》95：127-134，2011 年。
Circular dichroism (CD) spectroscopy of proteins/peptides in thin films can provide valuable information on the structures in the aggregated states; however, it is difficult to estimate the secondary structure content quantitatively due to artifact signals arising from macroscopic anisotropies which is unique to the solid phase. Using a Universal Chiroptical Spectrophotometer (UCS-1) together with the measurement and analytical procedures we have developed, we could obtain artifact-free CD spectra of cast and Langmuir-Blodgett (L-B) films of synthetic peptides, Ab (1-40) and (1-42) which are related to Alzheimer's disease. The work gave insights into the mechanisms for structural transformation and amyloid-like aggregation. (C) 2010 Wiley Periodicals, Inc. Biopolymers 95: 127-134, 2011.