Relationship between Polyamine Content and Ethylene Evolution During the Ripening of Japanese Pear 'Kosui'
Relationship between Polyamine Content and Ethylene Evolution During the Ripening of Japanese Pear 'Kosui'
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Kosui日本梨成熟过程中多胺含量与乙烯释放的关系
DOI：
10.2503/jjshs.67.970
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发表时间：
1998
影响因子：
--
通讯作者：
N. Hirata
中科院分区：
文献类型：
--
作者：
K. Ohkawa;H. Ohara;H. Matsui;E. Takahashi;N. Hirata
探讨日本梨果实成熟过程中多胺含量与乙烯释放的关系。采前1个月左右喷施高斯水、氨基氧乙酸(AOA)或甲基乙醛双鸟苷(MGBG)处理完好果实。处理后果实的乙烯释放及其1-氨基环丙烷-1-羧酸(ACC)和多胺含量的变化与对照进行了比较。5 mM AOA处理能阻止S-腺苷蛋氨酸(SAM)向ACC的转化，在收获前降低了乙烯释放量，尽管ACC含量几乎没有变化。这种处理在20d内逐渐增加了精胺(Spd)和精胺(Spm)的含量，随后它们的含量下降，同时在成熟期伴随着较低的腐胺(Put)水平。1 mM MGBG处理阻止了SAM向SPD或SPM的转化，在采收前显著增加了乙烯释放和ACC含量。处理后20d，PUT含量增加，Spd和Spm含量有降低趋势。这些结果表明，在‘kosui’果实成熟过程中，以SAM为底物的乙烯和多胺生物合成之间存在竞争关系。
To elucidate the relationship of polyamine content to ethylene evolution in fruit ripening of Japanese pear cv. Kosui, aminooxyacetic acid (AOA) or methylglyoxal bis-(guanylhydrazone) (MGBG) was administered to intact fruits about one month before harvest. After treatment, changes in ethylene evolution from the fruits and their 1-aminocyclopropane-1-carboxylic acid (ACC) and polyamine contents were compared to those of the control. Treatment with 5mM AOA, which is known to block the conversion of S-adenosyl-methionine (SAM) to ACC, decreased ethylene evolution just before harvest, although ACC content hardly changed. This treatment gradually increased spermidine (SPD) and spermine (SPM) contents for 20 days after which they subsequently decreased, accompanied by lower levels of putrescine (PUT) during ripening. Treatment with 1mM MGBG, which blocks the conversion of SAM to SPD or SPM, sharply increased ethylene evolution and ACC content just before harvest. The treatment also increased PUT content for 20 days after treatment and tended to lower SPD and SPM contents. These results indicate that a competitive relationship exists between ethylene and polyamine biosyntheses, using SAM as a substrate during the ripening process of 'Kosui' fruit.