Genetic diversity among silkworm (Bombyx mori L., Lep., Bombycidae) germplasms revealed by microsatellites
Genetic diversity among silkworm (Bombyx mori L., Lep., Bombycidae) germplasms revealed by microsatellites
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作者：
Li, MW;Shen, L;Huang, YP
为了明确家蚕品系间的遗传关系，利用SSR标记对31个不同来源、不同世代数、不同蜕皮次数和不同形态特征的菌株进行了研究。分析了家蚕基因组中微卫星侧翼序列的26对引物。所有这些都是多态性的，并且明确地将家蚕品系彼此分开。共检测到188个等位基因，平均值为7.2个等位基因/位点（范围2-17）。每个SSR位点的平均杂合度在0 ~ 0.60之间，最高杂合度为0.96（4013个位点中有1710516个位点）。平均多态性指数含量（PIC）为0.66（范围0.12-0.89）。用UPGMA法对家蚕品种按来源进行Nei遗传距离聚类分析。分析了7个主要家蚕生态类群。主成分分析（PCA）支持UPGMA聚类。结果表明，SSR标记是家蚕品种指纹图谱和遗传多样性研究的有效工具。
To determine genetic relationships among strains of silkworm, Bombyx mori L., 31 strains with different origins, number of generations per year, number of molts per generation, and morphological characters were studied using simple sequence repeat (SSR) markers. Twenty-six primer pairs flanking microsatellite sequences in the silkworm genome were assayed. All were polymorphic and unambiguously separated silkworm strains from each other. A total of 188 alleles were detected with a mean value of 7.2 alleles/locus (range 2-17). The average heterozygosity value for each SSR locus ranged from 0 to 0.60, and the highest one was 0.96 (1710516 in 4013). The mean polymorphism index content (PIC) was 0.66 (range 0.12-0.89). Unweighted pair group method with arithmetic means (UPGMA) cluster analysis of Nei's genetic distance grouped silkworm strains based on their origin. Seven major ecotypic silkworm groups were analyzed. Principal components analysis (PCA) for SSR data support their UPGMA clustering. The results indicated that SSR markers are an efficient tool for fingerprinting cultivars and conducting genetic-diversity studies in the silkworm.