STREPTOCOCCUS-FAECALIS SEX-PHEROMONE (CAM373) ALSO PRODUCED BY STAPHYLOCOCCUS-AUREUS AND IDENTIFICATION OF A CONJUGATIVE TRANSPOSON (TN918)
STREPTOCOCCUS-FAECALIS SEX-PHEROMONE (CAM373) ALSO PRODUCED BY STAPHYLOCOCCUS-AUREUS AND IDENTIFICATION OF A CONJUGATIVE TRANSPOSON (TN918)
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DOI：
10.1128/jb.162.3.1212-1220.1985
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发表时间：
1985-01-01
影响因子：
3.2
通讯作者：
GAWRONBURKE, C
中科院分区：
文献类型：
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作者：
CLEWELL, DB;AN, FY;GAWRONBURKE, C
粪链霉菌RC73含有一个接合质粒(PAM373)，它对同种无质粒成员分泌的性信息素(CAM373)产生交配反应。在所有23株金黄色葡萄球菌的培养滤液中也检测到信息素，但在22株凝固酶阴性葡萄球菌中只有2株检测到信息素。血链球菌和G9B菌株也能产生这种活性，但其他10株血链球菌不产生这种活性。该活性也由粪链霉菌9790产生。粪链球菌RC73中存在的一个四环素抗性(TC)决定簇与PAM373无关，但在从该菌株中存在的其他质粒中鉴定PAM373时，它是一个有用的标记。对T_c决定簇的分析表明，它位于一个与Tn916非常相似的接合转座子上。该转座子被命名为Tn918，可插入到pAM373和另外两个溶血素质粒中。虽然PAM373的衍生物在粪链球菌菌株之间很好地转移，但该质粒不能在金黄色葡萄球菌或血链球菌中建立。携带Tn918的PAM373的衍生物被证明是一种有用的载体，用于在葡萄球菌染色体上的多个位置产生转座子插入。
S. faecalis RC73 harbors a conjugative plasmid (pAM373) which confers a mating response to a sex pheromone (cAM373) excreted by plasmid-free members of the same species. The pheromone was also detected in culture filtrates of all of 23 S. aureus strains, but in only 2 of 22 coagulase negative staphylococcus strains. S. sanguis Challis and G9B also produced the activity, but 10 other S. sanguis strains did not. The activity was also produced by S. faecium 9790. A tetracycline resistance (Tc) determinant present in S. faecalis RC73 was not associated with pAM373 but served as a useful marker in efforts to identify pAM373 among other plasmids present in the strain. Analyses of the Tc determinant showed that it was located on a conjugative transposon very similar to Tn916. Designated Tn918, the transposon could insert into pAM373 and into 2 other hemolysin plasmids. Whereas pAM373 derivatives transferred very well between strains of S. faecalis, the plasmid would not establish in S. aureus or S. sanguis. A derivative of pAM373 carrying Tn918 proved to be a useful delivery vehicle for generating transposon insertions into multiple sites on the staphylococcal chromosome.