Chitosan particles agglomerated scaffolds for cartilage and osteochondral tissue engineering approaches with adipose tissue derived stem cells
Chitosan particles agglomerated scaffolds for cartilage and osteochondral tissue engineering approaches with adipose tissue derived stem cells
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DOI：
10.1007/s10856-005-4709-4
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发表时间：
2005-12-01
影响因子：
3.7
通讯作者：
Reis, RL
中科院分区：
文献类型：
--
作者：
Malafaya, PB;Pedro, AJ;Reis, RL
人们普遍认为，如果细胞没有细胞外基质（ECM）替代品，自然组织再生是不可能发生的。为了实现这一目标，已经使用了几种不同的方法来生产各种3D支架，作为适合骨和软骨组织工程的人工ECM替代品。此外，骨软骨组织工程提出了新的挑战，因为为了实现成功的骨软骨构建，需要结合骨和软骨的支架和共培养要求。本文报道了一种基于壳聚糖颗粒聚集法制备软骨和骨软骨组织工程支架的新工艺路线。本文提出了广泛的表征，包括使用微计算机断层扫描（mu CT）进行形态学评估和使用图像处理软件构建的3D虚拟模型。还进行了机械和吸水特性分析，证明了所开发的支架在拟议应用中的潜力。细胞毒性试验表明，制备的壳聚糖颗粒凝聚支架对细胞无毒性作用。此外，骨软骨双层支架也可以发展。从人脂肪组织中分离的间充质干细胞进行了初步播种，目的是为骨软骨组织工程应用开发成软骨和成骨分化的解决方案。(C) 2005 b施普林格科技+商业传媒有限公司
It is well accepted that natural tissue regeneration is unlikely to occur if the cells are not supplied with an extracellular matrix (ECM) substitute. With this goal, several different methodologies have been used to produce a variety of 3D scaffolds as artificial ECM substitutes suitable for bone and cartilage tissue engineering. Furthermore, osteochondral tissue engineering presents new challenges since the combination of scaffolding and co-culture requirements from both bone and cartilage applications is required in order to achieve a successful osteochondral construct.In this paper, an innovative processing route based on a chitosan particles aggregation methodology for the production of cartilage and osteochondral tissue engineering scaffolds is reported. An extensive characterization is presented including a morphological evaluation using Micro-Computed Tomography (mu CT) and 3D virtual models built with an image processing software. Mechanical and water uptake characterizations were also carried out, evidencing the potential of the developed scaffolds for the proposed applications. Cytotoxicity tests show that the developed chitosan particles agglomerated scaffolds do not exert toxic effects on cells. Furthermore, osteochondral bilayered scaffolds could also be developed. Preliminary seeding of mesenchymal stem cells isolated from human adipose tissue was performed aiming at developing solutions for chondrogenic and osteogenic differentiation for osteochondral tissue engineering applications. (C) 2005 Springer Science + Business Media, Inc.