Studies on transcriptional regulation of endogenous genes by ERF2 transcription factor in tobacco cells
Studies on transcriptional regulation of endogenous genes by ERF2 transcription factor in tobacco cells
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DOI：
10.1093/pcp/pcj017
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发表时间：
2006-04-01
影响因子：
4.9
通讯作者：
Shinshi, H
中科院分区：
文献类型：
--
作者：
Nakano, T;Nishiuchi, T;Shinshi, H
在这项研究中，我们发现乙烯应答转录因子（ERF） 2的过表达激活了烟草植物启动子区域具有GCC盒子的内源基因的表达。这些基因不仅包括防御相关基因CHN50（编码I类基本几丁质酶），还包括转录抑制基因ERF3。在糖皮质激素受体融合蛋白ERF2组成型表达的烟草植株中，地塞米松诱导ERF3 mRNA快速升高，CHN50 mRNA缓慢升高。这些结果表明，ERF2和ERF3的拮抗相互作用参与了烟草I类碱性几丁质酶基因的转录调控。
In this study, we showed that overexpression of ethylene-responsive transcription factor (ERF) 2 activated the expression of endogenous genes that have the GCC box in their promoter region, in tobacco plants. These include not only a defense-related gene, CHN50, encoding class I basic chitinase, but also a transcriptional repressor gene, ERF3. In tobacco plants constitutively expressing ERF2:glucocorticoid receptor fusion protein, treatment with dexamethazone induced a rapid increase of ERF3 mRNA and a slow increase of CHN50 mRNA. These results suggest that an antagonistic interplay of ERF2 and ERF3 is involved in the transcriptional regulation of the class I basic chitinase genes in tobacco.