Antiviral effect of Guazuma ulmifolia and Stryphnodendron adstringens on poliovirus and bovine herpesvirus
Antiviral effect of Guazuma ulmifolia and Stryphnodendron adstringens on poliovirus and bovine herpesvirus
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DOI：
10.1248/bpb.29.1092
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发表时间：
2006-06-01
影响因子：
2
通讯作者：
Nozawa, Carlos
中科院分区：
文献类型：
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作者：
Mestrimer Felipe, Adriana Meri;Rincao, Viniclus Pires;Nozawa, Carlos
杜鹃的粗提物(CE)、水提物(AQF)和乙酸乙酯(EtOAcF)组分，以及马兜铃(Stryphnode endron adstrens(Mart.)在HEp2细胞上检测了它们对脊髓灰质炎病毒1型(P-1)和牛疱疹病毒1型(BHV-1)的抗病毒活性。在杀毒和治疗方案下，用空斑试验和免疫荧光试验(IFA)检测其抗病毒活性。治疗方案对两种植物和两种病毒株都显示出统计上的阳性结果。对BHV-1和P-1复制的抑制率最高，分别为100%和99%(p<；0.05，学生t检验)。对土生链霉菌的抑制效果最好，对BHV-1和P-1的抑制率分别为97%和93%(p<；0.05)。在杀灭病毒方案中，银杏叶提取物对BHV-1和P-1病毒复制的抑制率分别为60%和26%(p<；0.05)，而对于加味葡萄球菌，仅乙氧AcF对P-1病毒复制的抑制率为62%(p<；0.05)。IFA显示，在两种病毒株的治疗方案下，荧光细胞数量的减少最大的是石杉。然而，在P-1的杀毒方案中，土豆根霉的AQF和EtOAcF的效果最好。综上所述，我们证明了石杉和土豆根霉抑制了BHV-1和P-1的复制，并阻断了感染细胞培养物中病毒抗原的合成。
Crude extract (CE) and aqueous (AqF) and ethyl acetate (EtOAcF) fractions of Guazuma ulmifolia LAM., Sterculiaceae and the corresponding AqF, EtOAcF of Stryphnodendron adstringens (MART.) COVILLE, Leguminosae were tested for their antiviral activity against poliovirus 1 (P-1) and bovine herpesvirus 1 (BHV-1) in HEp2 cultured cells. The antiviral activity was monitored by plaque assay and immunolluorescence assay (IFA) under virucidal and therapeutic protocols. The therapeutic protocol demonstrated statistically significant positive results with both plants and for both virus strains. The highest percentages of viral inhibition were found for G. ulmifolia EtOAcF which inhibited BHV-1 and P-1 replication by 100% and 99%, respectively (p < 0.05, Student's t-test). For S. adstringens, AqF was the most efficient, inhibiting BHV-1 and P-1 by 97% and 93%, respectively (p < 0.05). In the virucidal protocol, G. ulmifolia CE inhibited the replication of BHV-1 and P-1 by 60% and 26%, respectively (p < 0.05), while, for S. adstringens, inhibition of 62% (p < 0.05) was demonstrated only with EtOAcF for P-1. IFA demonstrated that the greatest reduction in fluorescent cell number occurred with G. ulmifolia, under the therapeutic protocol for both virus strains. However, AqF and EtOAcF of S. adstringens were most efficient with the virucidal protocol for P-1 In conclusion, we demonstrated that G. ulmifolia and S. adstringens inhibited BHV-1 and P-1 replication, as well as, blocked the synthesis of viral antigens in infected cell cultures.