Mechanical strain-induced extracellular matrix production by human vascular smooth muscle cells -: Role of TGF-β1
Mechanical strain-induced extracellular matrix production by human vascular smooth muscle cells -: Role of TGF-β1
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DOI：
10.1161/01.hyp.36.3.319
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发表时间：
2000-09-01
期刊：
影响因子：
8.3
通讯作者：
Williams, B
中科院分区：
文献类型：
--
作者：
O'Callaghan, CJ;Williams, B
血压升高会对血管壁施加更大的机械应力，而机械应变是血管平滑肌 (VSM) 细胞的有丝分裂刺激物。对于人类来源的 VSM 细胞来说，机械力在调节 VSM 细胞非细胞物质的产生中的作用仍不清楚。因此，我们研究了慢性周期性机械应变对培养的人 VSM 细胞产生细胞外基质 (ECM) 蛋白的影响。为了模拟 120/80 mm Hg 的血压，使用 Flexercell 装置将人类 VSM 细胞重复拉伸和放松其原始长度的 10% 至 16%。测量人类 VSM 细胞对机械应变的反应，纤连蛋白和胶原蛋白浓度、基质金属蛋白酶 (MMP) 活性以及转化生长因子-β(1) (TGF-β(1)) mRNA 表达。将人类 VSM 细胞暴露于 5 天的慢性周期性机械应变会增加纤连蛋白（+48%，P
Elevated blood pressure imposes increased mechanical stress on the vascular wall, and mechanical strain is a mitogenic stimulus for vascular smooth muscle (VSM) cells. The role of mechanical forces in regulating the production of noncellular material by VSM cells for VSM cells of human origin remains undefined. We thus investigated the effects of chronic cyclical mechanical strain on extracellular matrix (ECM) protein production by cultured human VSM cells. To simulate a blood pressure of 120/80 mm Hg, human VSM cells were repetitively stretched and relaxed by 10% to 16% of their original length with the Flexercell apparatus. Fibronectin and collagen protein concentrations, matrix metalloproteinase (MMP) activity, and transforming growth factor-beta(1) (TGF-beta(1)) mRNA expression by human VSM cells were measured in response to mechanical strain. Exposing human VSM cells to 5 days of chronic cyclical mechanical strain increased fibronectin (+48%, P