GluA1 trafficking and metabotropic NMDA: addressing results from other laboratories inconsistent with ours
GluA1 trafficking and metabotropic NMDA: addressing results from other laboratories inconsistent with ours
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DOI：
10.1098/rstb.2013.0145
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发表时间：
2014-01
期刊：
影响因子：
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通讯作者：
S. Nabavi;R. Fox;Stephanie Alfonso;Jonathan Aow;R. Malinow
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文献类型：
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作者：
S. Nabavi;R. Fox;Stephanie Alfonso;Jonathan Aow;R. Malinow
我们之前已经证明，当在神经元中过度表达时，绿色荧光蛋白（GFP）标记的GluA1 （GluA1-GFP）递送到突触依赖于可塑性。最近的一项研究表明，在缺乏额外的长期增强操作的情况下，GluA1过表达导致其并入突触。可能是GFP标签造成了这种差异。使用校正指数作为GluA1突触传递的测量，我们发现GluA1- gfp的突触传递与未标记的GluA1没有差异。我们最近发表的一项研究表明，虽然D-APV可以阻断nmda依赖的长期抑郁（LTD），但MK-801和7-氯基肌酸酯（7CK）不能阻断LTD。我们提出了NMDA受体在LTD诱导中的代谢功能。与我们的观察结果相反，最近未发表的数据表明，上述拮抗剂在阻断LTD方面同样有效。我们注意到他们的研究方法不同。在这里，我们表明他们的方法对突触传递有复杂的影响。因此，不可能得出结论，7CK是有效地阻止LTD从他们的实验类型。
We have previously shown that when over-expressed in neurons, green fluorescent protein (GFP) tagged GluA1 (GluA1-GFP) delivery into synapses is dependent on plasticity. A recent study suggests that GluA1 over-expression leads to its incorporation into the synapse, in the absence of additional long-term potentiation-like manipulations. It is possible that a GFP tag was responsible for the difference. Using rectification index as a measure of synaptic delivery of GluA1, we found no difference in the synaptic delivery of GluA1-GFP versus untagged GluA1. We recently published a study showing that while D-APV blocks NMDAr-dependent long-term depression (LTD), MK-801 and 7-chloro kynurenate (7CK) fail to block LTD. We propose a metabotropic function for the NMDA receptor in LTD induction. In contrast to our observations, recent unpublished data suggest that the above antagonists are equally effective in blocking LTD. We noticed different methodology in their study. Here, we show that their methodology has complex effects on synaptic transmission. Therefore, it is not possible to conclude that 7CK is effective in blocking LTD from their type of experiment.