Comparison of MicroRNA Expression in Tears of Normal Subjects and Sjogren Syndrome Patients
Comparison of MicroRNA Expression in Tears of Normal Subjects and Sjogren Syndrome Patients
复制标题
DOI：
10.1167/iovs.19-27062
复制
发表时间：
2019-11-01
影响因子：
4.4
通讯作者：
Kang, Min Ho
中科院分区：
文献类型：
--
作者：
Kim, Yu Jeong;Yeon, Yeji;Kang, Min Ho
目的.有报道称，在干燥综合征（SS）患者的血清或唾液腺中存在几种microRNA（miRNAs）的表达失调。然而，没有人研究过可以代表泪腺的样本中的miRNA。我们比较了SS患者和健康对照者泪液中的miRNAs表达。此外，我们还研究了miRNAs表达与眼染色评分（OSS）之间的相关性。从18名SS患者和8名年龄匹配的对照组中收集个人泪液样本。临床眼科评估包括Schirmer I试验、泪膜破裂时间（tBUT）和OSS。使用实时聚合酶链反应测量了泪液中43种不同miRNA的表达，并在SS患者和对照组之间进行了比较。我们还比较了对照组、原发性SS组和继发性SS组。评估miRNA表达与OSS之间的相关性。与对照组相比，SS患者中miR-16- 5 p、miR-34 a-5 p、miR-142- 3 p和miR-223- 3 p的表达水平显著上调（P <0. 05）。10种miRNAs（miR-30 b-5 p、miR-30 c-5 p、miR-30 d-5 p、miR-92 a-3 p、miR-134- 5 p、miR-137、miR-302 d-5 p、miR-365 b-3 p、miR-374 c-5 p、miR-487 b-3 p）在SS患者中的表达显著下调（P <0. 05）。8个miRNAs在对照组、原发性SS组和继发性SS组之间显示出统计学显著差异。SS组与对照组差异有统计学意义的14个miRNAs与OSS无明显相关性。这14个差异表达的miRNAs可能参与了SS的发病机制，尤其是与自身免疫和神经病变有关。
PURPOSE. Deregulated expression of several microRNAs (miRNAs) in sera or salivary glands of patients with Sjogren syndrome (SS) has been reported. However, none have investigated miRNAs in samples that can represent lacrimal glands. We compared the miRNAs expression in the tears of SS patients and healthy controls. Moreover, we investigated the correlation between miRNAs expression and ocular staining score (OSS).METHODS. Individual tear samples were collected from 18 SS patients and 8 age-matched controls. Clinical ophthalmologic assessments included Schirmer I test, tear film breakup time (tBUT), and OSS. The expression of 43 different miRNAs in tears was measured using real-time polymerase chain reaction, and compared between the SS patients and controls. And we also compared between the three groups of control, primary SS, and secondary SS patients. The correlation between the miRNA expression and OSS was evaluated.RESULTS. The expression levels of miR-16-5p, miR-34a-5p, miR-142-3p, and miR-223-3p were significantly upregulated in patients with SS when compared with those in the control group (P < 0.05). The expression of 10 miRNAs (miR-30b-5p, miR-30c-5p, miR-30d-5p, miR-92a-3p, miR-134-5p, miR-137, miR-302d-5p, miR-365b-3p, miR-374c-5p, miR-487b-3p) was significantly downregulated in the SS patients (P < 0.05). Eight miRNAs showed statistically significant differences between the three groups of control, primary SS and secondary SS. All 14 miRNAs with significant differences in SS patients and control group were not significantly correlated with OSSs.CONCLUSIONS. The 14 differentially expressed miRNAs may be involved in the pathogenesis of SS, in particular, related to autoinamunity and neuropathy.