Identification of ethanol responsive domains of adenylyl cyclase.
Identification of ethanol responsive domains of adenylyl cyclase.
复制标题
腺苷酸环化酶的乙醇反应域的鉴定。
DOI：
10.1111/j.1530-0277.2006.00219.x
复制
发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Gonzalez,CristinaE
中科院分区：
文献类型：
--
作者：
Yoshimura,Masami;Pearson,Susan;Kadota,Yoichi;Gonzalez,CristinaE
Background:The activity of adenylyl cyclase (AC) is enhanced by pharmacologically relevant concentrations of ethanol. The enhancing effect of ethanol on AC activity is AC isoform‐specific. Therefore, we hypothesized that within a cyclic AMP–generating system, AC is the target of ethanol's action and that ethanol‐sensitive AC molecules contain structural elements modulated by ethanol. The structural elements are designated as “ethanol responsive domains.”Methods:By using a series of chimeric mutants, we searched regions of the AC molecule that are important for the ethanol effect. These chimeric mutants were derived from 3 isoforms of AC: AC7 (type 7), the most ethanol responsive isoform; AC3 (type 3), an isoform that is far less responsive to ethanol; and AC2 (type 2), an isoform that is homologous to AC7 but less responsive to ethanol.Results:We identified 2 discrete regions of the AC molecule that are important for the enhancement of AC activity by ethanol. The first is the N‐terminal 28‐amino‐acid (aa) region of the C1adomain. The second is the C‐terminal region (∼140 aa) of the AC molecule. Sequence differences in the N‐terminal tail, 2 putative transmembrane domains, and the C1bdomain are not important for ethanol's effect.Conclusions:The current study with mammalian ACs provides a new class of alcohol‐responsive protein and possibly a new mechanism of alcohol action on cellular function. The identification of ethanol responsive domains will facilitate the elucidation of the mechanisms by which ethanol enhances the activity of AC.
