THE IDENTIFICATION OF HYDROXYMETHYLURACIL IN DNA OF TRYPANOSOMA-BRUCEI
THE IDENTIFICATION OF HYDROXYMETHYLURACIL IN DNA OF TRYPANOSOMA-BRUCEI
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DOI：
10.1093/nar/21.9.2039
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发表时间：
1993-05-11
影响因子：
14.9
通讯作者：
BORST, P
中科院分区：
文献类型：
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作者：
GOMMERSAMPT, JH;TEIXEIRA, AJR;BORST, P
我们先前已经报道了在布氏锥虫的DNA中检测到两种不寻常的核苷酸pdJ和pdV（Gommers-Ampt等人，1991年）。pdJ是一种新的核苷酸，可能参与调节锥虫特异性表面抗原基因的表达。最近的证据表明，V可能是J的前体，使V的生物合成和J的功能的研究中的一个关键化合物。因此，我们已经确定了V的结构，在这里，我们提出的证据，V是HOMeU。同一性基于dV（p）与真实HOMedU（p）的详细比较，显示：I）三种不同液相色谱分析中的共迁移II）相同的UV吸光度特征III）乙酰基-五氟苄基衍生化和随后的气相色谱/质谱（GC/MS）中的相同行为。GC/MS技术以前未用于分析从生物材料纯化的HOMedU。由于它的高灵敏度，它也可能是有用的检测由DNA的氧化损伤导致的低量的HOMedU。
We have previously reported the detection of two unusual nucleotides, pdJ and pdV, in the DNA of Trypanosoma brucei (Gommers-Ampt et al., 1991). pdJ was found to be a novel nucleotide and is possibly involved in the regulation of variant specific surface antigen gene expression in trypanosomes. Recent evidence suggests that V could be a precursor of J, making V a key compound in the study of the biosynthesis and function of J. We have therefore determined the structure of V and here we present proof that V is HOMeU. The identity is based on a detailed comparison of dV(p) with authentic HOMedU(p), showing: I) co-migration in three different liquid chromatography analyses II) identical UV absorbance characteristics III) identical behavior in acetyl-pentafluorobenzyl derivatization and subsequent Gas chromatography/Mass spectrometry (GC/MS). The GC/MS technique has not been used before to analyse HOMedU purified from biological material. Because of its high sensitivity, it may also be useful for the detection of the low amounts of HOMedU resulting from oxidative damage of DNA.