Reconstruct:: a free editor for serial section microscopy
Reconstruct:: a free editor for serial section microscopy
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DOI：
10.1111/j.1365-2818.2005.01466.x
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发表时间：
2005-04-01
期刊：
影响因子：
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通讯作者：
Fiala, JC
中科院分区：
文献类型：
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作者：
Fiala, JC
许多显微镜研究需要从连续切片重建，这是一种有时困难且耗时的分析方法。当每个切片被单独切割、安装和成像时，必须对切片图像进行拼接和重新对齐，以准确分析和可视化三维（3D）结构。重建是一个免费的编辑器，旨在促进蒙太奇，对齐，分析和可视化的连续部分。Reconstruct用于组织、转换和显示数据的方法通过有效利用计算机存储器，能够分析具有大量截面和大范围放大倍数图像的系列。对齐可以校正某些类型的非线性变形，包括裂缝和折叠，如在连续电子显微镜中经常遇到的。大量不同的结构可以很容易地跟踪和放置在一个单一的三维场景，可以动画或保存在一起。作为一种灵活的编辑器，Reconstruct可以减少连续切片研究所花费的时间和资源，并允许更快地分析更大的组织体积。
Many microscopy studies require reconstruction from serial sections, a method of analysis that is sometimes difficult and time-consuming. When each section is cut, mounted and imaged separately, section images must be montaged and realigned to accurately analyse and visualize the three-dimensional (3D) structure. Reconstruct is a free editor designed to facilitate montaging, alignment, analysis and visualization of serial sections. The methods used by Reconstruct for organizing, transforming and displaying data enable the analysis of series with large numbers of sections and images over a large range of magnifications by making efficient use of computer memory. Alignments can correct for some types of non-linear deformations, including cracks and folds, as often encountered in serial electron microscopy. A large number of different structures can be easily traced and placed together in a single 3D scene that can be animated or saved. As a flexible editor, Reconstruct can reduce the time and resources expended for serial section studies and allows a larger tissue volume to be analysed more quickly.