Selective overexpression of the QUTE gene encoding catabolic 3-dehydroquinase in multicopy transformants of Aspergillus nidulans.
Selective overexpression of the QUTE gene encoding catabolic 3-dehydroquinase in multicopy transformants of Aspergillus nidulans.
复制标题
编码分解代谢 3-脱氢喹酶的 QUTE 基因在构巢曲霉多拷贝转化体中选择性过表达。
DOI：
10.1042/bj2650337
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
A. Hawkins
中科院分区：
文献类型：
--
作者：
R. Beri;S. Grant;C. F. Roberts;M. Smith;A. Hawkins
将奎尼酸分解代谢为原儿茶酸所需的三种酶可由奎尼酸诱导产生，它们相应基因的转录受一个正调控激活基因和一个负调控阻遏基因的作用控制。含有多个激活基因（QUTA）拷贝但其他QUT基因只有单拷贝的构巢曲霉转化菌株，其基因簇保持正常调控，三种奎尼酸分解代谢酶没有任何过度表达。含有等量的激活基因（QUTA）和QUTE（编码分解代谢的3 - 脱氢奎尼酸酶）多个拷贝，但其他QUT基因只有单拷贝的转化菌株，其QUT基因簇保持正常调控，但在奎尼酸诱导时选择性地过度表达QUTE基因。所提供的数据有力地表明，基因QUTG在物理上位于QUT基因簇内，其功能尚未确定，它对于基因簇的表达不是必需的，也不编码绿原酸酯酶。
The three enzymes necessary to catabolize quinate to protocatechuate are inducible by quinic acid, and transcription of their corresponding genes is controlled by the action of a positively acting activator gene and a negatively acting repressor gene. Transformed strains of Aspergillus nidulans containing multiple copies of the activator gene (QUTA) but single copies of the other QUT genes retain normal regulation of the gene cluster and do not show any overexpression of the three quinic acid catabolic enzymes. Transformed strains containing equal multiple copies of the activator gene (QUTA) and QUTE (encoding catabolic 3-dehydroquinase), but single copies of the other QUT genes, retain normal regulation of the QUT gene cluster, but selectively overexpress the QUTE gene upon quinic acid induction. Data are presented that strongly suggested that the gene QUTG, which is physically located within the QUT gene cluster and for which no function has been identified, is not required for expression of the gene cluster and does not encode a chlorogenic acid esterase.