Differential intensity-dependent effects of pulsed electromagnetic fields on RANKL-induced osteoclast formation, apoptosis, and bone resorbing ability in RAW264.7 cells
Differential intensity-dependent effects of pulsed electromagnetic fields on RANKL-induced osteoclast formation, apoptosis, and bone resorbing ability in RAW264.7 cells
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脉冲电磁场对 RAW264.7 细胞中 RANKL 诱导的破骨细胞形成、凋亡和骨吸收能力的不同强度依赖性影响。
DOI：
10.1002/bem.22070
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发表时间：
2017-12-01
影响因子：
1.9
通讯作者：
Jing, Da
中科院分区：
文献类型：
--
作者：
Wang, Pan;Liu, Juan;Jing, Da
脉冲电磁场 (PEMF) 已被证明在实验和临床上可有效促进骨量和调节骨转换。然而，PEMF调节破骨细胞生成的确切机制以及PEMF光照射参数抑制破骨细胞活性和功能的确切机制仍不清楚，这对PEMF在临床中的广泛科学应用存在重大限制。在本研究中，将与 RANKL 一起孵育的 RAW264.7 细胞暴露于不同强度（0.5、1、2 和 3mT）的 15Hz PEMF（2 小时/天）中 7 天。我们证明，0.5mT PEMF 显着降低骨吸收能力，主要是通过抑制破骨细胞的形成和成熟，但在 3mT PEMF 时，通过促进破骨细胞凋亡而增强骨吸收能力。此外，0.5mT PEMF 使 RANK、NFATc1、TRAP、CTSK、BAX 和 BAX/BCL-2 的基因表达显着降低，但增加 3mT。我们的研究结果揭示了低强度脉冲电磁场在调节骨吸收方面具有显着的强度窗口，具有多种性质，可调节破骨细胞生成和细胞凋亡。该研究不仅丰富了我们对PEMF调节破骨细胞生成的基础知识，而且可能导致PEMF在调节骨转换和抑制骨质减少/骨质疏松方面更高效、更科学的临床应用。生物电磁学。 38:602-612，2017 年。(c) 2017 年 Wiley 期刊公司。
Pulsed electromagnetic fields (PEMF) have been proven to be effective for promoting bone mass and regulating bone turnover both experimentally and clinically. However, the exact mechanisms for the regulation of PEMF on osteoclastogenesis as well as optical exposure parameters of PEMF on inhibiting osteoclastic activities and functions remain unclear, representing significant limitations for extensive scientific application of PEMF in clinics. In this study, RAW264.7 cells incubated with RANKL were exposed to 15Hz PEMF (2h/day) at various intensities (0.5, 1, 2, and 3mT) for 7 days. We demonstrate that bone resorbing capacity was significantly decreased by 0.5mT PEMF mainly by inhibiting osteoclast formation and maturation, but enhanced at 3mT by promoting osteoclast apoptosis. Moreover, gene expression of RANK, NFATc1, TRAP, CTSK, BAX, and BAX/BCL-2 was significantly decreased by 0.5mT PEMF, but increased by 3mT. Our findings reveal a significant intensity window for low-intensity PEMF in regulating bone resorption with diverse nature for modulating osteoclastogenesis and apoptosis. This study not only enriches our basic knowledge for the regulation of PEMF in osteoclastogenesis, but also may lead to more efficient and scientific clinical application of PEMF in regulating bone turnover and inhibiting osteopenia/osteoporosis. Bioelectromagnetics. 38:602-612, 2017. (c) 2017 Wiley Periodicals, Inc.