Apigenin promotes osteogenic differentiation of mesenchymal stem cells and accelerates bone fracture healing via activating Wnt/I3-catenin signaling
Apigenin promotes osteogenic differentiation of mesenchymal stem cells and accelerates bone fracture healing via activating Wnt/I3-catenin signaling
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芹菜素通过激活 Wnt/I3-catenin 信号促进间充质干细胞成骨分化并加速骨折愈合
DOI：
10.1152/ajpendo.00543.2019
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Zhang Jin-fang
中科院分区：
文献类型：
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作者：
Pan Fei-fei;Shao Jiang;Shi Chuan-fian;Li Zhi-peng;Fu Wei-ming;Zhang Jin-fang
芹菜素（Apigenin， API）是一种天然的植物黄酮，在常见的水果和蔬菜中含量丰富。原料药是一种具有生物活性的类黄酮，具有抗增殖、抗炎症等多种活性。最近的一项研究表明，API可以延缓骨质疏松症的进展，表明其在骨骼系统中的作用。然而，其具体功能和机制尚不清楚。本研究发现API可促进间充质干细胞（MSCs）的成骨分化。进一步研究发现，API可增强Wnt信号通路关键转录因子β-catenin及多个下游靶基因的表达，从而激活Wnt/β-catenin信号通路。通过大鼠股骨骨折模型，发现API在体内促进新骨形成，加速骨折愈合。综上所述，我们的数据表明，API可以通过激活Wnt/β-catenin信号通路，在体外促进骨生成，在体内促进骨折愈合，表明API可能是一种有前景的骨折修复治疗候选药物。1) API促进人间充质干细胞体外成骨分化；2) API在体内促进骨形成，加速骨折愈合；3) API刺激人MSCs成骨过程中Wnt/β-catenin信号通路。
Apigenin (API), a natural plant flavone, is abundantly found in common fruits and vegetables. As a bioactive flavonoid, API exhibits several activities including antiproliferation and anti-inflammation. A recent study showed that API could retard osteoporosis progress, indicating its role in the skeletal system. However, the detailed function and mechanism remain obscure. In the present study, API was found to promote osteogenic differentiation of mesenchymal stem cells (MSCs). And further investigation showed that API could enhance the expression of the critical transcription factor β-catenin and several downstream target genes of Wnt signaling, thus activated Wnt/β-catenin signaling. Using a rat femoral fracture model, API was found to improve new bone formation and accelerate fracture healing in vivo. In conclusion, our data demonstrated that API could promote osteogenesis in vitro and facilitate the fracture healing in vivo via activating Wnt/β-catenin signaling, indicating that API may be a promising therapeutic candidate for bone fracture repair.NEW & NOTEWORTHY1) API promoted osteogenic differentiation of human MSCs in vitro;2) API facilitated bone formation and accelerated fracture healing in vivo;3) API stimulated Wnt/β-catenin signaling during osteogenesis of human MSCs.