ENHANCEMENT OF EXPERIMENTAL ULCERATIVE-COLITIS BY IMMUNIZATION WITH BACTEROIDES-VULGATUS
ENHANCEMENT OF EXPERIMENTAL ULCERATIVE-COLITIS BY IMMUNIZATION WITH BACTEROIDES-VULGATUS
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DOI：
10.1128/iai.42.2.783-788.1983
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发表时间：
1983-01-01
影响因子：
3.1
通讯作者：
BRONSON, RT
中科院分区：
文献类型：
--
作者：
ONDERDONK, AB;CISNEROS, RL;BRONSON, RT
先前对豚鼠溃疡性结肠炎模型的研究表明，诱导剂角叉菜胶不会在无菌豚鼠中引起溃疡，但动物与B单相关。当喂食角叉菜胶时，普通鸡产生盲肠溃疡。为了定义B的角色。在该模型系统中，用B免疫常规豚鼠。在卡拉胶处理之前的普通番茄。免疫动物产生循环抗体和阳性皮肤试验的同源抗原。比较B。在角叉菜胶治疗30天后，普通免疫和非免疫角叉菜胶接受者在两组中均显示盲肠和大肠溃疡，但免疫组比非免疫组发生更严重的损伤。在B中检测到最严重的肠道病变。普通免疫动物同时喂食角叉菜胶和每日剂量的活B。普通人B的免疫受体。单独的普通犬没有发生溃疡，但显示出肠道炎症的组织学证据。在B中观察到病变。与在非免疫角叉菜胶接受者中检测到的更严重的盲肠损伤相比，普通免疫动物的结肠和直肠损伤最严重。用B进行类似的实验。脆弱性作为免疫抗原在免疫组和非免疫组之间没有差异。这些结果表明，用B. vulgatus增强角叉菜胶诱导的结肠炎的严重性。
Previous studies with the guinea pig model for ulcerative colitis have shown that the inducing agent, carrageenan, does not provoke ulcerations in germfree guinea pigs, but animals monoassociated with B. vulgatus develop cecal ulcerations when fed carrageenan. In an effort to define the role of B. vulgatus in this model system, conventional guinea pigs were immunized with B. vulgatus before carrageenan treatment. Immunized animals developed both circulating antibody and positive skin tests for the homologous antigen. A comparison of B. vulgatus-immune and nonimmune carrageenan recipients after 30 days of carrageenan treatment revealed ulcerations of the ceca and large intestine in both groups, but the immune group developed more severe lesions than the nonimmune group. The most severe intestinal lesions were detected in B. vulgatus-immune animals fed both carrageenan and daily doses of viable B. vulgatus. Immune recipients of B. vulgatus alone did not develop ulcerations, but showed histological evidence of intestinal inflammation. Lesions observed in B. vulgatus-immune animals were most severe in the colon and rectum, in contrast to more severe cecal lesions detected in nonimmune carrageenan recipients. Similar experiments with B. fragilis as an antigen for immunization showed no difference between immune and nonimmune groups. These results suggest that immunization with B. vulgatus enhances the severity of carrageenan-induced colitis.