Independent responses to ultraviolet radiation and herbivore attack in broccoli
Independent responses to ultraviolet radiation and herbivore attack in broccoli
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DOI：
10.1093/jxb/erp182
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发表时间：
2009-08-01
影响因子：
6.9
通讯作者：
Mueller, Caroline
中科院分区：
文献类型：
--
作者：
Kuhlmann, Franziska;Mueller, Caroline
植物对紫外线b辐射（UV-B）的反应和对昆虫食草的反应被认为是部分相似的。在本研究中，研究了西兰花（Brassica oleracea L. convar）对这些因素的响应。葡萄孢属十字花科)。植物首先在三种UV-B环境（80%，23%和4%的环境UV-B透过率）下种植，温室覆盖创新材料（高和中等透过率）或传统玻璃（低透过率）。然后，一半的植物在这些条件下保持不变，但另一半被转移到具有环境光和草食植物通道的田地中长达3天。通过分析放置在室内和暴露在田间的植物的形态和化学参数，研究了植物对不同环境条件的反应。此外，还研究了田间暴露植物对天然昆虫的适宜性与UV-B预处理的关系。高水平的UV-B辐射导致西兰花类黄酮浓度增加，但生物量积累降低。这些模式在户外暴露后仍然存在。然而，紫外线诱导的植物性状变化并没有改变植物对食草昆虫的吸引力：蓟马、粉虱和蚜虫独立于UV-B预处理攻击植物。所有植株地上部组织中吲哚基硫代葡萄糖苷浓度增加了3倍，这很可能是由于田间暴露3天后大量食草动物的攻击。结果表明，植物对不同的非生物和生物影响具有高特异性，表明植物对胁迫因子的感知和加工是分开的。
The plant responses to ultraviolet-B radiation (UV-B) and to insect herbivory are believed to be partially similar. In this study, responses to these factors were investigated in the crop species broccoli (Brassica oleracea L. convar. botrytis, Brassicaceae). Plants were first grown under three UV-B regimes (80%, 23%, and 4% transmittance of ambient UV-B) in greenhouses covered with either innovative materials (high and medium transmittance) or conventional glass (low transmittance). Half of the plants then remained under these conditions, but the other half were transferred to the field with ambient light and herbivore access for up to 3 d. The plant responses to distinct environmental conditions were examined by analysing the morphological and chemical parameters of plants kept inside and plants exposed in the field. Furthermore, suitability of field-exposed plants to naturally occurring insects was investigated in relation to UV-B pretreatment. High levels of UV-B radiation led to increased flavonoid concentrations, but to a lower biomass accumulation in broccoli. These patterns remained after outdoor exposure. However, UV-induced changes of plant traits did not alter attractiveness to herbivorous insects: thrips, whiteflies, and aphids attacked plants independently of UV-B pretreatment. A 3-fold increase of indolyl glucosinolate concentrations occurred in above-ground tissue of all the plants, most likely due to massive herbivore attack after 3 d of field exposure. The results show that plants respond with high specificity to different abiotic and biotic impacts, demonstrating the separate perception and processing of stress factors.