No-Go Zones for Mariner Transposition.
No-Go Zones for Mariner Transposition.
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DOI：
10.1128/mbio.01690-16
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发表时间：
2016-10-11
期刊：
影响因子：
6.4
通讯作者：
Akerley BJ
中科院分区：
文献类型：
--
作者：
Akerley BJ
转座子随机插入靶DNA的特性长期以来一直被用于广义诱变和正向遗传筛选。监测每个转座子插入在大型突变体库中的相对丰度的较新应用可以用于评估生物体的所有基因在细胞适应性中的作用，只要转座在所有基因中实际上是随机的。在最近的一篇文章中，Kimura和他的同事发现了后一种假设的一个重要例外[S。木村，T. P. Hubbard，B. M.戴维斯，米。K. Waldor，mBio 7（4）：e01351-16，2016，doi：10.1128/mBio.01351-16]。他们提供的证据表明，水手转座子在组蛋白样类核结构蛋白H-NS的存在下表现出基因座特异性位点偏好。这种效应被证明会使霍乱弧菌中重要毒力位点的结果产生偏差，并导致体外生长相关基因的错误鉴定。幸运的是，这种细菌的大部分基因组不受这种偏差的影响，并且识别H-NS效应允许过滤以提高结果的准确性。
The property of transposons to randomly insert into target DNA has long been exploited for generalized mutagenesis and forward genetic screens. Newer applications that monitor the relative abundance of each transposon insertion in large libraries of mutants can be used to evaluate the roles in cellular fitness of all genes of an organism, provided that transposition is in fact random across all genes. In a recent article, Kimura and colleagues identified an important exception to the latter assumption [S. Kimura, T. P. Hubbard, B. M. Davis, M. K. Waldor, mBio 7(4):e01351-16, 2016, doi:10.1128/mBio.01351-16]. They provide evidence that the Mariner transposon exhibits locus-specific site preferences in the presence of the histone-like nucleoid structuring protein H-NS. This effect was shown to bias results for important virulence loci in Vibrio cholerae and to result in misidentification of genes involved in growth in vitro. Fortunately, the bulk of this bacterium’s genome was unaffected by this bias, and recognizing the H-NS effect allows filtering to improve the accuracy of the results.