8-Prenyl daidzein and 8-prenyl genistein from germinated soybean modulate inflammatory response in activated macrophages
8-Prenyl daidzein and 8-prenyl genistein from germinated soybean modulate inflammatory response in activated macrophages
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DOI：
10.1093/bbb/zbad041
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发表时间：
2023-05-01
影响因子：
1.6
通讯作者：
Goto，Tsuyoshi
中科院分区：
文献类型：
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作者：
Jheng，Huei-Fen;Takase，Miho;Goto，Tsuyoshi
大豆异黄酮已被证明具有抗炎特性;然而，大豆发芽过程中产生的异黄酮代谢物的抗炎作用仍不清楚。我们发现大豆黄酮和染料木黄酮衍生物8-异戊烯基大豆黄酮（8-PD）和8-异戊烯基染料木黄酮（8-PG）在抑制巨噬细胞炎症反应方面表现出比大豆黄酮和染料木黄酮更强的作用。尽管Ik B蛋白水平没有改变，但8-PD和8-PG抑制了核因子κ B（NF-κ B B）的活化，这与ERK 1/2、JNK和p38 MAPK活化的减少有关，并抑制了促分裂原和应激激活激酶1的磷酸化。8-PD和8-PG处理成功地抑制了由含有肥大脂肪细胞分泌物的培养基诱导的炎症反应。在体外研究中，8-PD和8-PG显著抑制长期高脂饮食小鼠脂肪组织中促炎C-C基序趋化因子配体2（CCL 2）的分泌。这些数据表明，8-PD和8-PG可以调节肥胖条件下的巨噬细胞活化。
Soy isoflavones have been shown to have anti-inflammatory properties; however, the anti-inflammatory effects of isoflavone metabolites produced during soybean germination remain unclear. We found that the daidzein and genistein derivatives, 8-prenyl daidzein (8-PD) and 8-prenyl genistein (8-PG), demonstrated a more potent effect than daidzein and genistein on repressing inflammatory responses in macrophages. Although IkB protein levels were unaltered, 8-PD and 8-PG repressed nuclear factor kappa B (NF-κB) activation, which was associated with reduced ERK1/2, JNK, and p38 MAPK activation and suppressed mitogen- and stress-activated kinase 1 phosphorylation. Inflammatory responses induced by the medium containing hypertrophic adipocyte secretions were successfully suppressed by 8-PD and 8-PG treatment. In theex vivostudy, 8-PD and 8-PG significantly inhibited proinflammatory C–C motif chemokine ligand 2 (CCL2) secretion from the adipose tissues of mice fed a long-term high-fat diet. The data suggest that 8-PD and 8-PG could regulate macrophage activation under obesity conditions.