Aeromonas hydrophila isolated from food and drinking water: Hemagglutination, hemolysis, and cytotoxicity for a human intestinal cell line (HT-29)
Aeromonas hydrophila isolated from food and drinking water: Hemagglutination, hemolysis, and cytotoxicity for a human intestinal cell line (HT-29)
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DOI：
10.1128/aem.62.9.3459-3461.1996
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发表时间：
1996-09-01
影响因子：
4.4
通讯作者：
Letarte, R
中科院分区：
文献类型：
--
作者：
Handfield, M;Simard, P;Letarte, R
对从食品和饮用水中分离的嗜水气单胞菌进行了溶血性、血凝性和细胞毒性试验。溶血，测试红细胞从六个不同的物种，更频繁地看到与水分离株（64%）比与食物分离株（48%），血凝更频繁地遇到与食物分离株（92%）比与水分离株（73%）。在7个细胞系上评价的细胞毒性经常在食物分离株（92%）和水分离株（73%）中观察到。培养上清液的热处理（56 ℃，10分钟）抑制了一些但不是所有产毒分离株的毒性。我们的研究结果表明，人肠细胞系HT-29可能是一个有用的补充测试A。用于研究该物种对人类的肠道致病性。
Aeromonas hydrophila isolated from food and drinking water was tested for pathogenicity by studying its hemolysis, hemagglutination, and cytotoxicity. Hemolysis, tested on erythrocytes from six different species, was more frequently seen with water isolates (64%) than with food isolates (48%), Hemagglutination was more frequently encountered with food isolates (92%) than with water isolates (73%). Cytotoxicity, evaluated on seven cell lines, was frequently observed with food isolates (92%) and with water isolates (73%). Heat treatment (56 degrees C for 10 min) of culture supernatant fluids inhibited the toxicity of some but not all toxin-producing isolates. Our results suggest that the human intestinal cell line HT-29 could be a useful complement for testing A. hydrophila exotoxins and for studying the enteropathogenicity of this species for humans.