Efficient adsorption of hemoglobin from aqueous solutions by hybrid monolithic cryogel column
Efficient adsorption of hemoglobin from aqueous solutions by hybrid monolithic cryogel column
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DOI：
10.1016/j.msec.2016.12.036
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发表时间：
2017-04-01
影响因子：
7.9
通讯作者：
Odabasi, Mehmet
中科院分区：
文献类型：
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作者：
Baran, Nuray Yilmaz;Acet, Omur;Odabasi, Mehmet
以聚甲基丙烯酸羟乙酯（PHEMA）为载体，N，N '-亚甲基双丙烯酰胺（MBAAm）为交联剂，在Cu ~（2+）-ABPs存在下，通过自由基低温共聚合制备了一种超大孔Cu ~（2+）-膨润土包埋杂化单块晶凝胶（Cu ~（2+）-ABPs EHMC），并将其用于血红蛋白（Hb）的分离。由于膨润土表面天然存在大量的羟基，负载Cu 2+的膨润土颗粒（Cu 2 +-ABPs）包埋杂化整体晶凝胶（HMC）对血红蛋白（Hb）具有良好的吸附性能，在pH 6.0的磷酸盐缓冲溶液中，流速为0.5 mL/min时，吸附容量可达521.6 mg/g膨润土，吸附稳定性好，吸附时间可达60 min，具有良好的选择性和可逆性。与其他蛋白质分离吸附剂体系相比，该吸附剂具有吸附性能优异、制备简单、适用、高效、环境友好等优点，是一种可持续发展的蛋白质分离纯化材料。(C)© 2016 Elsevier B. V.版权所有。
In this study, a supermacroporous poly(2-hydroxyethyl methacrylate) (PHEMA)-based Cu2+-attached bentonite particles embedded hybrid monolithic cryogel (Cu2+-ABPs EHMC) was prepared by radical cryo-copolymerization of HEMA with N,N'-methylene-bis-acrylamide (MBAAm) as a cross-linker directly in a plastic syringe in the presence of Cu2+-ABPs, and used for hemoglobin (Hb) separation from aqueous solution. Due to the naturally abundant hydroxyl groups on bentonite, Cu2+-attached bentonite particles (Cu2+-ABPs) embedded hybrid monolithic cryogel (HMC) showed a good adsorption performance for Hb, covering an excellent adsorption capacity of 521.6 mg/g bentonite in phosphate buffer at pH 6.0 with a flow rate of 0.5 mL/min, fast binding stability within 60 min, well selectivity and reversibility. Compared with other adsorbent systems Used for protein separation, this sorbent has many advantages such as excellent adsorption performance, ease of preparation, suitable, effective and environment-friendly to perform, which can serve as a more sustainable material in protein separation and purification. (C) 2016 Elsevier B.V. All rights reserved.