The tRNA-Tyr gene family of Saccharomyces cerevisiae: agents of phenotypic variation and position effects on mutation frequency.
The tRNA-Tyr gene family of Saccharomyces cerevisiae: agents of phenotypic variation and position effects on mutation frequency.
复制标题
酿酒酵母的 tRNA-Tyr 基因家族：表型变异的因子和位置对突变频率的影响。
DOI：
10.1093/genetics/161.4.1395
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发表时间：
2002
期刊：
影响因子：
3.3
通讯作者：
McCusker,JohnH
中科院分区：
文献类型：
--
作者：
Ito-Harashima,Sayoko;Hartzog,PhillipE;Sinha,Himanshu;McCusker,JohnH
Extensive phenotypic diversity or variation exists in clonal populations of microorganisms and is thought to play a role in adaptation to novel environments. This phenotypic variation or instability, which occurs by multiple mechanisms, may be a form of cellular differentiation and a stochastic means for modulating gene expression. This work dissects a case of phenotypic variation in a clinically derivedSaccharomyces cerevisiaestrain involving acox15ochre mutation, which acts as a reporter. The ochre mutation reverts to sense at a low frequency while tRNA-Tyr ochre suppressors (SUP-o) arise at a very high frequency to produce this phenotypic variation. TheSUP-omutations are highly pleiotropic. In addition, although allSUP-omutations within the eight-member tRNA-Tyr gene family suppress the ochre mutation reporter, there are considerable phenotypic differences among the differentSUP-omutants. Finally, and of particular interest, there is a strong position effect on mutation frequency within the eight-member tRNA-Tyr gene family, with one locus,SUP6, mutating at a much higher than average frequency and two other loci,SUP2andSUP8, mutating at much lower than average frequencies. Mechanisms for the position effect on mutation frequency are evaluated.
