Isolation and expression analysis of the testis-specific gene, human OPPO1
Isolation and expression analysis of the testis-specific gene, human OPPO1
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DOI：
10.1023/b:jarg.0000029497.30205.cf
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发表时间：
2004-04-01
影响因子：
3.1
通讯作者：
Ishikawa, M
中科院分区：
文献类型：
--
作者：
Miyamoto, T;Sengoku, K;Ishikawa, M
目的：为了研究人类精子发生，我们分离了人类睾丸特异的基因。方法：利用小鼠的氨基酸序列，在人类基因组序列中找到包含氨基酸水平同源性的区域。制备含内含子的引物，进行RT-PCR和RACE分析。结果：获得了人OPPO1的全长cDNA。它编码257个氨基酸残基。人OPPO1主要在睾丸中表达。另一方面，ZNF8、GR194、GR219、GR093、GR046、GR163和GR200的部分基因在不同组织中均有表达。
Purpose: To investigate human spermatogenesis, we isolated human testis-specific genes.Methods: Using mouse amino acid sequences, we found the region including homology in amino acid level in the human genome sequences. The primers encompassing introns were made and RT-PCR and RACE were carried out. The resultant PCR products were sequenced.Results: The full-length cDNA of human OPPO1 was isolated. It encodes 257 amino acid residues. The expression of the human OPPO1 was predominantly in the testis. On the other hand, partial cDNAs of ZNF8, GR194, GR219, GR093, GR046, GR163, and GR200 were expressed in the various tissues.Conclusions: Our data suggests that the human OPPO1 may play important roles in human spermatogenesis.