Detection of flowing fluorescent particles in a microcapillary using fluorescence correlation spectroscopy
Detection of flowing fluorescent particles in a microcapillary using fluorescence correlation spectroscopy
复制标题
DOI：
10.1021/ac0256742
复制
发表时间：
2002-10-15
影响因子：
7.4
通讯作者：
Visser, AJWG
中科院分区：
文献类型：
--
作者：
Kunst, BH;Schots, A;Visser, AJWG
使用荧光相关光谱作为分析工具，描述了荧光分子、细菌和微球的毛细管流动实验。微毛细管中的流速是通过将自相关迹线与包含与扩散和流动相关的参数的模型拟合来确定的。微毛细管内压力驱动流的流动剖面是通过使用小染料分子的荧光波动来确定的。研究发现，激光束产生的光力会阻碍细菌和微球的流动。
Capillary flow experiments are described with fluorescent molecules, bacteria, and microspheres using fluorescence correlation spectroscopy as an analytical tool. The flow velocity in the microcapillary is determined by fitting autocorrelation traces with a model containing parameters related to diffusion and flow. The flow profile of pressure-driven flow inside a microcapillary is determined by using the fluorescence fluctuations of a small dye molecule. It was found that bacteria and microspheres are retarded in their flow by optical forces produced by the laser beam.