Identification of promoter exchange at a male fertility restorer locus for cytoplasmic male sterility in radish (Raphanus sativus L.)
Identification of promoter exchange at a male fertility restorer locus for cytoplasmic male sterility in radish (Raphanus sativus L.)
复制标题
萝卜细胞质雄性不育雄性育性恢复基因座启动子交换的鉴定（Raphanus sativus L.）
DOI：
10.1007/s11032-017-0683-z
复制
发表时间：
2017-06-01
影响因子：
3.1
通讯作者：
Wang, Ting
中科院分区：
文献类型：
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作者：
Wang, Zhi Wei;De Wang, Chuan;Wang, Ting
细胞质雄性不育（CMS）/核控育性恢复（Rf）系统是利用作物杂种优势的主要组成部分。在这里，我们已经确定了一个新的五肽重复序列（PPR）编码等位基因在RsRf 3基因座连接到不育恢复萝卜。该等位基因被命名为RsRf 3 -5，编码一个具有18个PPR和预测的线粒体靶向信号的推定蛋白。另一个等位基因RsRf 3 -6编码与RsRf 3 -5相同的蛋白，具有不同的启动子区域，并且不能恢复CMS的育性。编码具有15个PPR的预测蛋白的非恢复等位基因RsRf 3 -7具有与RsRf 3 -6相同的启动子区，表明RsRf 3 -6可能是由RsRf 3 -5和RsRf 3 -7之间的最近交叉产生的。有趣的是，与RsRf 3 -6等位基因相比，RsRf 3 -5等位基因在CMS细胞质背景中显示出更高的RNA表达水平。
The cytoplasmic male sterility (CMS)/nucleus-controlled fertility restoration (Rf) system is a major component that exploits hybrid vigor in crops. Here, we have identified a new pentatricopeptide repeat (PPR)-encoding allele at the RsRf3 locus linked to the fertility restoration to CMS in radish. This allele was named as RsRf3-5 and encodes a putative protein with 18 PPRs and a predicted mitochondrial targeting signal. Another allele RsRf3-6 encoding the same protein with RsRf3-5 possesses a different promoter region and has failed to restore fertility to CMS. The non-restoring allele RsRf3-7 encoding a predicted protein with 15 PPRs has an identical promoter region with RsRf3-6, suggesting that RsRf3-6 could result from a recent crossover between RsRf3-5 and RsRf3-7. Interestingly, the RsRf3-5 allele shows higher RNA expression levels in the CMS cytoplasmic background compared with the RsRf3-6 allele.