Facile incorporation of REDV into porous silk fibroin scaffolds for enhancing vascularization of thick tissues
Facile incorporation of REDV into porous silk fibroin scaffolds for enhancing vascularization of thick tissues
复制标题
轻松将 REDV 掺入多孔丝素蛋白支架中，以增强厚组织的血管化
DOI：
10.1016/j.msec.2018.07.062
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发表时间：
2018-12-01
影响因子：
7.9
通讯作者：
Fan, Yubo
中科院分区：
文献类型：
--
作者：
Yao, Danyu;Qian, Zhiyong;Fan, Yubo
支架内的快速新生血管化对于厚的复杂组织的再生至关重要。已经探索了肽和其他活性分子的表面固定以改善植入物的血管化能力。然而，这些分子的快速降解、反应条件和交联通常导致血管化能力降低。在这里，我们介绍了一个温和的，全水的过程，以实现散装多孔丝素蛋白（SF）支架。采用温度控制的方法，通过自组装的方法，诱导出水稳定结构。在自组装过程中，将Arg-Glu-Asp-Val（REDV）肽加入SF溶液中并固定在SF支架内。结果表明，功能化支架在体外能显著促进内皮细胞的粘附。此外，体内研究表明，在植入后4周，在本体功能化SF支架中增强的细胞浸润和令人印象深刻的血管化。功能化支架表现出优异的血管化能力，为厚组织再生提供了令人兴奋的生物材料选择。
Rapid neovascularization within scaffolds is critical for the regeneration of thick complex tissues. The surface immobilization of peptides and other active molecules have been explored to improve the vascularization capacity of implants. However, the rapid degradation of these molecules, the reaction conditions and cross-linking usually result in decreased vascularization capability. Here, we introduced a temperate, all-aqueous process to achieve bulk porous silk fibroin (SF) scaffolds. A temperature controlled process was used to induce the water stable structure by SF self-assembly. Arg-Glu-Asp-Val (REDV) peptides were added into SF solution and fixed within SF scaffolds during the self-assembly process. The results showed that the functionalized scaffolds markedly promoted the adhesion of endothelial cells in vitro. Moreover, the in vivo studies indicated enhanced cell infiltration in the bulk functionalized SF scaffolds and impressive vaseularization at 4 weeks post-implantation. The functionalized scaffolds demonstrated excellent vascularization capability, providing an exciting biomaterial option for thick tissue regeneration.