BIOSYNTHESIS OF ACETYLCHOLINE IN TURTLE PHOTORECEPTORS
BIOSYNTHESIS OF ACETYLCHOLINE IN TURTLE PHOTORECEPTORS
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DOI：
10.1073/pnas.69.7.1987
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发表时间：
1972-01-01
影响因子：
11.1
通讯作者：
LAM, DMK
中科院分区：
文献类型：
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作者：
LAM, DMK
为了确定可能由感光细胞合成的神经递质，海龟视网膜被解离成单细胞与蛋白水解酶。这些细胞通过速度沉降部分分离，以产生富含光感受器的级分。然后将单个感光细胞吸入微量移液管中，并与已知或疑似神经递质的标记前体一起孵育。孵育后，通过高压电泳分析放射性产物。在所有测试的化学物质中，海龟感光细胞只合成乙酰胆碱，这表明这些细胞可能是胆碱能的。
For determination of possible neurotransmitters synthesized by photoreceptor cells, turtle retinas were dissociated into single cells with proteolytic enzymes. These cells were partially separated by velocity sedimentation to yield a fraction rich in photoreceptors. Individual photoreceptor cells were then sucked into a micropipette and incubated with labeled precursors of known or suspected neurotransmitters. After incubation, the radioactive products were analyzed by high-voltage electrophoresis. Of all the chemicals tested, turtle photoreceptor cells synthesized only acetylcholine, suggesting that these cells may be cholinergic.