Esophageal cancer phospholipid characterization by 31P NMR.
Esophageal cancer phospholipid characterization by 31P NMR.
复制标题
通过 31P NMR 表征食管癌磷脂。
DOI：
10.1002/nbm.1940060304
复制
发表时间：
1993
影响因子：
2.9
通讯作者：
Glonek,T
中科院分区：
文献类型：
--
作者：
Merchant,TE;deGraaf,PW;Minsky,BD;Obertop,H;Glonek,T
本文用~（31）P核磁共振法分析了18例正常食管、食管远端肿瘤和正常胃手术切除组织的磷脂提取物。在这些组织中检测到的主要磷脂包括心磷脂（CL）、磷脂酰乙醇胺缩醛原、磷脂酰乙醇胺（PE）、磷脂酰丝氨酸（PS）、鞘磷脂（SPH）、磷脂酰肌醇（PI）、磷脂酰胆碱缩醛原和磷脂酰胆碱（PC）。在分析的54个组织标本中，也检测到极少量的磷脂溶血磷脂酰胆碱、磷脂酸、磷脂酰甘油和-0.13 δ的未表征磷脂。这些磷脂的平均相对浓度，摩尔百分比的总检测到的磷，确定从所获得的光谱，并用于区分三个组织组。以下磷脂的相对浓度在各组织组之间存在显著差异（p<0.001）：正常食管与食管肿瘤，PS、SPH、PI、PC;正常食管与正常胃，CL、PE、PS、SPH;食管肿瘤与正常胃，CL、PE。利用~（31）P核磁共振技术，可以对与调节上皮源性肿瘤的生长和转移有关的膜磷脂进行分析，以区分正常食管、远端食管肿瘤和正常胃。
Phospholipid extracts of surgical tissue specimens from 18 patients, consisting of normal esophagus, distal esophageal tumor and normal stomach, were analyzed using31P NMR. The prominent phospholipids detected in these tissues included cardiolipin (CL), phosphatidylethanolamine plasmalogen, phosphatidylethanolamine (PE), phosphatidylserine (PS), sphingomyelin (SPH), phosphatidylinositol (PI), phosphatidylcholine plasmalogen and phosphatidylcholine (PC). Very small quantities of the phospholipids lysophosphatidylcholine, phosphatidic acid, phosphatidylglycerol, and an uncharacterized phospholipid at −0.13 δ also were detected in some of the 54 tissue specimens analyzed. The mean relative concentrations of these phospholipids, in mole percentages of total detected phosphorus, were determined from the acquired spectra and used to differentiate among the three tissue groups. The relative concentrations of the following phospholipids differed significantly (p<0.001) among the respective tissue groups: normal esophagus vs esophageal tumor, PS, SPH, PI, PC; normal esophagus vs normal stomach, CL, PE, PS, SPH; esophageal tumor vs normal stomach, CL, PE. Membrane phospholipids implicated in modulating the growth and metastases of tumors of epithelial origin can be profiled to discriminate among normal esophagus, distal esophageal tumor and normal stomach using31P NMR.