Mutant of Escherichia coli with Derepressed Levels of the Biotin Biosynthetic Enzymes
Mutant of Escherichia coli with Derepressed Levels of the Biotin Biosynthetic Enzymes
复制标题
生物素生物合成酶水平降低的大肠杆菌突变体
DOI：
10.1128/jb.112.3.1280-1287.1972
复制
发表时间：
1972
影响因子：
3.2
通讯作者：
C. Pai
中科院分区：
文献类型：
--
作者：
C. Pai
使用交叉饲喂技术分离出大肠杆菌 K-12 突变体，该突变体向生长培养基中分泌的生物素比亲本菌株多 1,000 倍。即使在存在生物素的情况下生长，该突变体也具有高水平的生物素生物合成酶。脱硫生物素合成酶的水平被用作生物素途径的生物合成活性的量度，在足以抑制野生型中该酶的浓度的250,000倍的浓度下，生物素不会抑制脱硫生物素合成酶。该突变基因可与位于大肠杆菌染色体上 77 分钟处的 argC 共转导。
A cross-feeding technique was used to isolate a mutant of Escherichia coli K-12 that excretes 1,000 times more biotin into the growth medium than the parent strain. The mutant has high levels of the biotin biosynthetic enzymes even when grown in the presence of biotin. Desthiobiotin synthetase, the level of which was used as a measure of the biosynthetic activity of the biotin pathway, is not repressed by biotin at the concentration 250,000 times that sufficient to repress the enzyme in the wild type. The mutant gene is cotransducible with argC located at 77 min on the E. coli chromosome.