The characteristic expression of B7-H3 and B7-H4 in liver biopsies from patients with HBV-related acute-on-chronic liver failure
The characteristic expression of B7-H3 and B7-H4 in liver biopsies from patients with HBV-related acute-on-chronic liver failure
复制标题
B7-H3 和 B7-H4 在 HBV 相关慢加急性肝衰竭患者肝活检中的特征性表达。
DOI：
10.1111/j.1440-1827.2012.02856.x
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发表时间：
2012-10-01
影响因子：
2.2
通讯作者：
Chen, Yongwen
中科院分区：
文献类型：
--
作者：
Guo, Guoning;Cao, Dayan;Chen, Yongwen
乙型肝炎病毒（HBV）是一个主要的公共卫生问题，而乙型肝炎相关的慢性肝衰竭（HBV-ACLF）由于缺乏有效的治疗方法而预后极差。 B7-H3 和 B7-H4 是 B7 超家族的两个新成员，积极参与调节传染病的发病机制。然而，这两个成员在 HBV-ACLF 患者的肝内表达尚未得到描述。在本研究中，我们通过免疫组织化学分析了 HBV-ACLF 活检中 B7-H3 和 B7-H4 的表达。我们的结果显示，在所有 HBV-ACLF 样本中均观察到这两个成员，并且它们的表达主要在浸润的炎症细胞和受损的胆管上观察到。免疫荧光双染结果显示，B7-H4主要表达于CD3+T细胞、CD68+巨噬细胞、CK-18+胆管和CD31+内皮细胞，而B7-H3在所有检测到的细胞类型中均有表达。在这些肝组织中还检测到程序性死亡（PD）-1配体PD-L1和PD-L2的表达，并且发现它们与B7-H3和B7-H4共表达。这些结果表明B7家族信号最有可能影响这种疾病的发病机制，清楚地了解它们的功能作用可能会进一步阐明疾病过程。
Hepatitis B virus (HBV) is a major public health problem, and HBV-related acute-on-chronic liver failure (HBV-ACLF) has an extremely poor prognosis due to a lack of effective treatments. B7-H3 and B7-H4 are two novel members of the B7 superfamily that are actively involved in regulating the pathogenesis of infectious diseases. However, the intrahepatic expression of both members in HBV-ACLF patients has yet to be described. In this study, we analyzed the expression of B7-H3 and B7-H4 in HBV-ACLF biopsies by immunohistochemistry. Our results showed that both members were observed in all HBV-ACLF samples, and their expression was chiefly observed on infiltrating inflammatory cells and the damaged bile ducts. Immunofluorescence double staining showed that B7-H4 was expressed chiefly on CD3+ T cells, CD68+ macrophages, CK-18+ bile ducts, and CD31+ endothelial cells, while B7-H3 was found on all cell types detected. The expression of the programmed death (PD)-1 ligands, PD-L1 and PD-L2, was also detected in these liver tissues and they were found to be co-expressed with B7-H3 and B7-H4. These results suggest that the B7-family signaling is most likely to affect the pathogenesis of this disease, and a clear understanding of their functional roles may further elucidate the disease process.