Lipid-Protein Interactions Are Unique Fingerprints for Membrane Proteins
Lipid-Protein Interactions Are Unique Fingerprints for Membrane Proteins
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DOI：
10.1021/acscentsci.8b00143
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发表时间：
2018-06-27
影响因子：
18.2
通讯作者：
Tieleman, D. Peter
中科院分区：
文献类型：
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作者：
Corradi, Valentina;Mendez-Villuendas, Eduardo;Tieleman, D. Peter
细胞膜含有数百种不对称排列的不同蛋白质和脂质。我们目前对细胞膜详细组织的理解仍然相当难以捉摸，因为以所需的时空分辨率研究脂质和蛋白质的波动纳米级组装体是一项挑战。在这里，我们使用分子动力学模拟来表征 10 种不同膜蛋白的脂质环境。为了提供真实的脂质环境，蛋白质被嵌入模型质膜中，其中代表了 60 多种脂质种类，不对称地分布在小叶之间。模拟详细说明了每种蛋白质如何通过特定脂质成分的富集或消耗，以独特的方式调节其局部脂质环境，从而产生厚度和曲率梯度。我们的结果提供了脂质-蛋白质相互作用复杂性的分子视角，对于我们理解真实细胞膜的整体组织具有潜在深远的影响。
Cell membranes contain hundreds of different proteins and lipids in an asymmetric arrangement. Our current understanding of the detailed organization of cell membranes remains rather elusive, because of the challenge to study fluctuating nanoscale assemblies of lipids and proteins with the required spatiotemporal resolution. Here, we use molecular dynamics simulations to characterize the lipid environment of 10 different membrane proteins. To provide a realistic lipid environment, the proteins are embedded in a model plasma membrane, where more than 60 lipid species are represented, asymmetrically distributed between the leaflets. The simulations detail how each protein modulates its local lipid environment in a unique way, through enrichment or depletion of specific lipid components, resulting in thickness and curvature gradients. Our results provide a molecular glimpse of the complexity of lipid-protein interactions, with potentially far-reaching implications for our understanding of the overall organization of real cell membranes.