Isolation and characterization of the porcine hypothalamic growth hormone releasing factor.
Isolation and characterization of the porcine hypothalamic growth hormone releasing factor.
复制标题
猪下丘脑生长激素释放因子的分离和表征。
DOI：
10.1016/0006-291x(83)90585-5
复制
发表时间：
1983
影响因子：
3.1
通讯作者：
Guillemin,R
中科院分区：
文献类型：
--
作者：
Böhlen,P;Esch,F;Brazeau,P;Ling,N;Guillemin,R
从2500头猪下丘脑中分离得到一种由44个氨基酸组成的内源性生长激素释放肽，该肽具有较高的内源性生长激素释放活性。生长激素释放因子的结构特征是通过气相序列分析的完整肽和其羧基末端溴化氰消化片段。天然肽和合成复制品的反相液相色谱显示，该分子在其羧基末端具有酰胺而不是游离酸。肽的结构确定为：Tyr-Ala-Asp-Ala-Ile-Phe-Thr-Asn-Ser-Tyr-Arg-Lys-Val-Leu-Gly-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Met-Ser-Arg-Gln-Gln-Gly-Glu-Arg-Asn-Gln-Glu-Gln-Gly-Ala-Arg-Val-Arg-Leu-NH 2，使用约6 nmol的材料。
A 44 amino acid peptide with high intrinsic growth hormone releasing activity was isolated from 2500 porcine hypothalami by means of acid extraction, immunoaffinity chromatography, gel filtration, and 2 steps of reverse phase HPLC. The growth hormone releasing factor was structurally characterized by gas phase sequence analyses of the intact peptide and its carboxyl terminal cyanogen bromide digestion fragment. Reverse phase liquid chromatography of the native peptide and synthetic replicates showed that the molecule possesses an amide rather than a free acid at its carboxyl terminus. The structure of the peptide was established as: Tyr-Ala-Asp-Ala-Ile-Phe-Thr-Asn-Ser-Tyr-Arg-Lys-Val-Leu-Gly-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp- Ile-Met-Ser-Arg-Gln-Gln-Gly-Glu-Arg-Asn-Gln-Glu-Gln-Gly-Ala-Arg-Val-Arg-Leu-NH2using approximately 6 nmol of material.