Fluorescence detection of hydroxyl radicals
Fluorescence detection of hydroxyl radicals
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DOI：
10.1016/j.radphyschem.2005.10.011
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发表时间：
2006-04-01
影响因子：
2.9
通讯作者：
Milligan, JR
中科院分区：
文献类型：
--
作者：
Newton, GL;Milligan, JR
羟基自由基（（中心点）01-1）是水辐射分解的产物，与羟基化芳香有机化合物反应。在某些情况下，这些羟基化产物是荧光的。例如苯甲酸酯、香豆素和苯恶嗪酮系统。对于这些体系的代表性成员，我们确定了反应的速率常数。OH和荧光产物的产率。速率常数均在2 × 10(9) ~ 2 × 10(10) L mol(-1) s（-1）之间，每个(OH)- o中心点的产率为5- 11%。这些结果表明，构建一个由两个基团组成的探针是可行的，这两个基团都必须与(OH)- o中心点反应才能产生荧光。荧光共振能量转移的高效过程意味着这种探针可能能够检测到*OH簇，这通常被认为是电离辐射能量沉积的特征。(c) 2005 Elsevier Ltd版权所有。
The hydroxyl radical ((center dot)01-1), a product of water radiolysis, reacts to hydroxylate aromatic organic compounds. In some cases, these hydroxylated products are fluorescent. Examples include the benzoate, coumarin, and phenoxazinone systems. For representative members of these systems, we have determined both the rate constants for reaction with. OH and the yields of the fluorescent products. The rate constants all fall in the range 2 x 10(9) to 2 x 10(10) L mol(-1) s(-1), and the yields 5-11 % per (OH)-O-center dot. These results Suggest that it may prove feasible to construct a probe consisting of two groups both of which must react with (OH)-O-center dot to become fluorescent. The efficient process of fluorescence resonance energy transfer implies that such a probe might be able to detect *OH clusters, which are generally assumed to be a characteristic feature of energy deposition by ionizing radiation. (c) 2005 Elsevier Ltd. All rights reserved.