Mast cells play an important role in chlamydia pneumoniae lung infection by facilitating immune cell recruitment into the airway.
Mast cells play an important role in chlamydia pneumoniae lung infection by facilitating immune cell recruitment into the airway.
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DOI：
10.4049/jimmunol.1402685
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发表时间：
2015-04-15
期刊：
影响因子：
--
通讯作者：
Arditi M
中科院分区：
文献类型：
--
作者：
Chiba N;Shimada K;Chen S;Jones HD;Alsabeh R;Slepenkin AV;Peterson E;Crother TR;Arditi M
肥大细胞被认为是过敏和过敏反应的核心参与者，在宿主防御某些病原体方面发挥着关键作用。肺炎衣原体（Cpn）是一种重要的人类病原体，但目前尚不清楚肥大细胞在Cpn感染过程中发挥什么作用。我们用 Cpn 感染了 C57BL/6 (WT) 和肥大细胞缺陷小鼠 Kitw-sh/w-sh (Wsh)。 Wsh 小鼠比 WT 小鼠表现出更高的存活率，尽管细胞因子和趋化因子水平相似，但 Wsh BALF 中的细胞较少。我们还发现，与 WT 小鼠相比，Wsh 小鼠肺部的细菌清除速度更快。 Cromolyn 是一种肥大细胞稳定剂，可减少 BAL 细胞和细菌负担，与 Wsh 小鼠类似；相反，化合物 48/80（一种肥大细胞脱颗粒剂）会增加 BAL 细胞的数量和细菌负荷。组织学显示WT小鼠肺部有弥漫性炎症，而Wsh小鼠则有中性粒细胞斑片状积聚和淋巴细胞血管周围积聚。受感染的 Wsh 小鼠 BALF 中 MMP-9 的量减少，并且对上皮整合膜蛋白降解具有抵抗力，表明 Wsh 小鼠中屏障完整性保持完整。 Wsh 小鼠的肥大细胞重建导致细菌生长增强和正常的上皮整合膜蛋白降解，突出了肥大细胞在该模型中的特殊作用。这些数据表明，肥大细胞在 Cpn 感染过程中发挥着有害作用，因为它促进免疫细胞渗入空气空间并为 Cpn 提供更有利的复制环境。
Mast cells are known as central players in allergy and anaphylaxis, and play a pivotal role in host defense against certain pathogens. Chlamydia pneumoniae (Cpn) is an important human pathogen, but it is unclear what role mast cells play during Cpn infection. We infected C57BL/6 (WT) and mast cell-deficient mice, Kitw-sh/w-sh (Wsh), with Cpn. Wsh mice showed improved survival than WT, with fewer cells in Wsh BALF despite similar levels of cytokines and chemokines. We also found a more rapid clearance of bacteria from the lungs of Wsh mice compared with WT. Cromolyn, a mast cell stabilizer, reduced BAL cells and bacterial burden similar to Wsh mice; conversely, Compound 48/80, a mast cell degranulator, increased the number of BAL cells and bacterial burden. Histology showed that WT lungs had diffuse inflammation while Wsh mice had patchy accumulations of neutrophils and perivascular accumulations of lymphocytes. Infected Wsh mice had reduced amounts of MMP-9 in BALF and were resistant to epithelial integral membrane protein degradation, suggesting that barrier integrity remains intact in Wsh mice. Mast cell reconstitution in Wsh mice led to enhanced bacterial growth and normal epithelial integral membrane protein degradation, highlighting the specific role of mast cells in this model. These data suggest that mast cells play a detrimental role during Cpn infection by facilitating immune cell infiltration into the airspace and providing a more favorable replicative environment for Cpn.
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DOI：
10.1084/jem.20101757
发表时间：
2011-08-29
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Föger N;Jenckel A;Orinska Z;Lee KH;Chan AC;Bulfone-Paus S
通讯作者：
Bulfone-Paus S
DOI：
10.4049/jimmunol.0903937
发表时间：
2010-05-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
He X;Mekasha S;Mavrogiorgos N;Fitzgerald KA;Lien E;Ingalls RR
通讯作者：
Ingalls RR
影响因子：
64.8
作者：
Malaviya, R;Ikeda, T;Abraham, SN
通讯作者：
Abraham, SN
DOI：
10.1007/s10096-011-1530-5
发表时间：
2012-08-01
影响因子：
4.5
作者：
Le, B. V.;Khorsi-Cauet, H.;Gay-Queheillard, J.
通讯作者：
Gay-Queheillard, J.
DOI：
10.1073/pnas.0912551107
发表时间：
2010-05-11
影响因子：
11.1
作者：
Dietrich, Nicole;Rohde, Manfred;Gekara, Nelson O.
通讯作者：
Gekara, Nelson O.