Two roles for aconitase in the regulation of tricarboxylic acid branch gene expression in Bacillus subtilis.
Two roles for aconitase in the regulation of tricarboxylic acid branch gene expression in Bacillus subtilis.
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乌头酸酶在枯草芽孢杆菌三羧酸分支基因表达调节中的两个作用。
DOI：
10.1128/jb.01690-12
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发表时间：
2013
影响因子：
3.2
通讯作者：
Sonenshein,AbrahamL
中科院分区：
文献类型：
--
作者：
Pechter,KieranB;Meyer,FrederikM;Serio,AlisaW;Stulke,Jorg;Sonenshein,AbrahamL
Previously, it was shown that an aconitase (citB) null mutation results in a vast overaccumulation of citrate in the culture fluid of growing Bacillus subtilis cells, a phenotype that causes secondary effects, including the hyperexpression of thecitBpromoter. B. subtilis aconitase is a bifunctional protein; to determine if either or both activities of aconitase were responsible for this phenotype, two strains producing different mutant forms of aconitase were constructed, one designed to be enzymatically inactive (C450S [citB2]) and the other designed to be defective in RNA binding (R741E [citB7]). ThecitB2mutant was a glutamate auxotroph and accumulated citrate, while thecitB7mutant was a glutamate prototroph. Unexpectedly, thecitB7strain also accumulated citrate. Both mutant strains exhibited overexpression of thecitBpromoter and accumulated high levels of aconitase protein. These strains and thecitBnull mutant also exhibited increased levels of citrate synthase protein and enzyme activity in cell extracts, and the major citrate synthase (citZ) transcript was present at higher-than-normal levels in thecitBnull mutant, due at least in part to a >3-fold increase in the stability of thecitZtranscript compared to the wild type. Purified B. subtilis aconitase bound to thecitZ5′ leader RNAin vitro, but the mutant proteins did not. Together, these data suggest that wild-type aconitase binds to and destabilizes thecitZtranscript in order to maintain proper cell homeostasis by preventing the overaccumulation of citrate.
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影响因子：
5.6
作者：
Jourlin-Castelli, C;Mani, N;Sonenshein, AL
通讯作者：
Sonenshein, AL
DOI：
10.1073/pnas.96.18.10412
发表时间：
1999-08-31
影响因子：
11.1
作者：
Alén, C;Sonenshein, AL
通讯作者：
Sonenshein, AL
两个不同的枯草芽孢杆菌柠檬酸合酶基因的鉴定
影响因子：
3.2
作者：
Shengfang Jin;A. Sonenshein
通讯作者：
A. Sonenshein
铁在乌头酸酶激活-失活中的作用。
影响因子：
4.8
作者：
M. Kennedy;Mark H. Emptageg;Jean;H. Beinert
通讯作者：
H. Beinert
“1972政权”日本安保体系下的台湾（中文）
DOI：
--
发表时间：
2022
期刊：
影响因子：
--
作者：
Barrett;B;Ohta;H.;and Trencher;G. "Prospects for Acceleration of Socio-Technical Transitions for Deep Decarbonization," pp. 400-414;青山瑠妙;福田円;舒旻「中国：EUとの脆弱な相互依存」pp. 247-271;福田円;青山瑠妙;青山瑠妙;青山瑠妙;福田円;青山瑠妙;福田円
通讯作者：
福田円