Antimicrobial Probiotics Reduce Salmonella enterica in Turkey Gastrointestinal Tracts.
Antimicrobial Probiotics Reduce Salmonella enterica in Turkey Gastrointestinal Tracts.
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DOI：
10.1038/srep40695
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发表时间：
2017-01-17
影响因子：
4.6
通讯作者：
Kaznessis YN
中科院分区：
文献类型：
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作者：
Forkus B;Ritter S;Vlysidis M;Geldart K;Kaznessis YN
尽管采用了一系列技术来控制食源性非伤寒沙门氏菌（NTS），但数十年来感染率并未下降。家禽是NTS疫情的主要来源，也是全球增长最快的肉类行业。随着最近FDA在动物生产中逐步淘汰抗生素的规定，开发新的病原体减少策略的压力越来越大。我们报告了一种减少家禽沙门氏菌的技术。我们设计了益生菌E. coli Nissle 1917，表达并分泌抗菌肽Microcin J25。利用体外实验和300只火鸡的动物模型，我们建立了这项技术的疗效。沙门氏菌比其他处理组更快地清除施用修饰的益生菌的鸟的盲肠。在沙门氏菌攻击后14天，在处理组中测量到沙门氏菌携带降低约97%。益生菌通常被认为是安全的消费，是胆汁抗性的，并且可以被修饰以产生现在已知的全套抗微生物肽。报告的系统可能为平台提供基础，以推出针对胃肠道病原体的抗菌药物，包括多重耐药性病原体。
Despite the arsenal of technologies employed to control foodborne nontyphoidal Salmonella (NTS), infections have not declined in decades. Poultry is the primary source of NTS outbreaks, as well as the fastest growing meat sector worldwide. With recent FDA rules for phasing-out antibiotics in animal production, pressure is mounting to develop new pathogen reduction strategies. We report on a technology to reduce Salmonella enteritidis in poultry. We engineered probiotic E. coli Nissle 1917, to express and secrete the antimicrobial peptide, Microcin J25. Using in vitro experiments and an animal model of 300 turkeys, we establish the efficacy of this technology. Salmonella more rapidly clear the ceca of birds administered the modified probiotic than other treatment groups. Approximately 97% lower Salmonella carriage is measured in a treated group, 14 days post-Salmonella challenge. Probiotic bacteria are generally regarded as safe to consume, are bile-resistant and can plausibly be modified to produce a panoply of antimicrobial peptides now known. The reported systems may provide a foundation for platforms to launch antimicrobials against gastrointestinal tract pathogens, including ones that are multi-drug resistant.