A Novel Method for the Assay of α-Glucosidase Inhibitory Activity Using a Multi-channel Oxygen Sensor
A Novel Method for the Assay of α-Glucosidase Inhibitory Activity Using a Multi-channel Oxygen Sensor
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使用多通道氧传感器测定 α-葡萄糖苷酶抑制活性的新方法
DOI：
10.2116/analsci.18.1315
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发表时间：
2002
影响因子：
1.6
通讯作者：
H. Katayama
中科院分区：
文献类型：
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作者：
Kiyoshi Matsumoto;Kayoko Takemata;K. Takayama;K. J. M. Abesundara;T. Matsui;H. Katayama
采用固定化葡萄糖氧化酶（GOD）和变旋酶（MUT）的多通道溶解氧（DO）仪（DOX 96）对葡萄糖进行定量。通过改进我们的批式假体内测定系统评价α -葡糖苷酶（AGH）的抑制活性[Oki等; Biol. Pharm. Bull.，2000，232，1084]也使用DOX 96进行。当45 U/孔GOD和18.75 U/孔MUT固定在电极上的明胶膜表面上时，由同一行中的8个电极威尔斯获得的DO（%）的减少百分比所示的响应与葡萄糖浓度直到3.3 mM呈线性，并且相关系数大于0.9。为了估计AGH抑制活性，使用沉默筛选板的孔中的AGH固定化琼脂糖凝胶支持物。阿卡波糖和糖尿病药物抑制剂1-脱氧野尻霉素的IC_50值分别为0.70 ± 0.08 µM和0.40 ± 0.13 µM，与伪体内试验结果吻合良好。
The quantification of glucose by using a multi-channel dissolved oxygen (DO) meter (DOX96) with immobilized glucose oxidase (GOD) and mutarotase (MUT) was performed. An evaluation of the inhibitory activities for α -glucosidase (AGH) by modifying our batch-type pseudo- in vivo assay system [Oki et al .; Biol. Pharm. Bull. , 2000 , 232 , 1084] was also performed using a DOX96. When 45 U/well GOD and 18.75 U/well MUT were immobilized on the surface of a gelatin membrane on the electrodes, the response shown by the decrease percent of DO (%) obtained with 8 electrode wells in the same row was linear with the glucose concentration up to 3.3 mM and a correlation coefficient larger than 0.9. To estimate the AGH inhibitory activity, AGH-immobilized Sepharose supports in the well of a silent screen plate were used. The IC_50 values of acarbose and 1-deoxynojirimycin, a medicinal inhibitor for diabetes, were 0.70 ± 0.08 µM and 0.40 ± 0.13 µM, respectively, and coincided well with those by a pseudo- in vivo assay.
葡萄糖生成抑制剂
DOI：
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发表时间：
2022
期刊：
影响因子：
--
作者：
通讯作者：
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