On-chip wavelength multiplexed detection of cancer DNA biomarkers in blood
On-chip wavelength multiplexed detection of cancer DNA biomarkers in blood
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DOI：
10.1063/1.4968033
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发表时间：
2016-11-01
期刊：
影响因子：
3.2
通讯作者：
Schmidt, H.
中科院分区：
文献类型：
--
作者：
Cai, H.;Stott, M. A.;Schmidt, H.
我们开发了一种光流控分析系统，可以处理从全血开始的生物分子样本，然后在硅基分子检测平台上分析和识别多个目标。我们使用可编程微流体电路演示了血液过滤、样品提取、目标富集和荧光标记。我们使用基于反谐振反射光波导芯片上的波长相关多点激发的光谱复用技术来检测和识别多个目标。具体来说，我们从全血中提取两种类型的黑色素瘤生物标志物：突变的无细胞核酸 -BRAFV600E 和 NRAS。我们使用光谱复用方法同时检测和识别这两个目标，成功率高达 96%。这些结果为实现复杂生物样品的高性能分析的完整的基于从前到后芯片的光流控紧凑系统指明了道路。由 AIP 出版社出版。
We have developed an optofluidic analysis system that processes biomolecular samples starting from whole blood and then analyzes and identifies multiple targets on a silicon-based molecular detection platform. We demonstrate blood filtration, sample extraction, target enrichment, and fluorescent labeling using programmable microfluidic circuits. We detect and identify multiple targets using a spectral multiplexing technique based on wavelength-dependent multi-spot excitation on an antiresonant reflecting optical waveguide chip. Specifically, we extract two types of melanoma biomarkers, mutated cell-free nucleic acids -BRAFV600E and NRAS, from whole blood. We detect and identify these two targets simultaneously using the spectral multiplexing approach with up to a 96% success rate. These results point the way toward a full front-to-back chip-based optofluidic compact system for high-performance analysis of complex biological samples. Published by AIP Publishing.