High-pressure liquid chromatography of cobalamins and cobalamin analogs.
High-pressure liquid chromatography of cobalamins and cobalamin analogs.
复制标题
钴胺素和钴胺素类似物的高压液相色谱法。
DOI：
10.1016/0003-2697(82)90003-3
复制
发表时间：
1982
影响因子：
2.9
通讯作者：
Allen,RH
中科院分区：
文献类型：
--
作者：
Binder,M;Kolhouse,JF;VanHorne,KC;Allen,RH
高压液相色谱已用于分离、鉴定和定量 37 种不同的氰钴胺类似物，包括由细菌合成产生的最常见的类似物。该技术还用于同时分离、鉴定和定量五种天然存在的钴胺素，它们的上轴配体不同：甲基钴胺素、腺苷钴胺素、羟钴胺素、氰钴胺素和硫钴胺素。该方法可以快速定量检测和鉴定复杂混合物中的钴胺素以及天然存在和合成的钴胺素类似物。
High-pressure liquid chromatography has been used to separate, identify, and quantitate 37 different cyanocobalamin analogs, including the most commonly occurring analogs that result from bacterial synthesis. This technique has also been used to simultaneously separate, identify, and quantitate five naturally occurring cobalamins that differ in their upper axial ligands: methylcobalamin, adenosylcobalamin, hydroxocobalamin, cyanocobalamin, and sulfitocobalamin. This method permits rapid quantitative detection and identification of cobalamins and naturally occurring and synthetic cobalamin analogs in complex mixtures.