Other Notable Methods of Membrane Protein Detection: A Brief Review.
Other Notable Methods of Membrane Protein Detection: A Brief Review.
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DOI：
10.1007/978-1-4939-2718-0_36
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Scofield RH
中科院分区：
文献类型：
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作者：
Kurien BT;Scofield RH
已经使用了几种方法来检测膜上的蛋白质。这些包括使用量子点发光标记、氧印迹免疫化学检测、聚合物免疫复合物、“偶联”探测方法、用于检测粗品或纯化制剂中酶活性的蛋白质原位复性、免疫色谱测定、蛋白质磷酸酶测定和使用刚果红染料，一种名为阿尔塔的化妆品颜色，Pro-Q Emerald 488染料或胺活性染料与碱性磷酸酶和辣根过氧化物酶抗体结合物的组合，用于蛋白质的同时三色荧光检测。已经使用了几种方法来改善膜上的蛋白质的检测，包括戊二醛处理的硝酸纤维素印迹，消除角蛋白伪影的免疫印迹探测与多克隆抗体和洗涤的免疫印迹用过量的水和操作的吐温-20在洗涤缓冲液。本章将简要介绍这些方法。
Several methods have been used for detecting proteins on membranes. These include the use of quantum dot luminescent labels, oxyblot immunochemical detection, polymer immunocomplexes, ‘coupled’ probing approach, in situ renaturation of proteins for detecting enzyme activities in crude or purified preparations, immunochromatographic assay, western-phosphatase assay and the use of Congo red dye, a cosmetic color named Alta, Pro-Q Emerald 488 dye or amine-reactive dye in combination with alkaline phosphatase-and horseradish peroxidase-antibody conjugates for the simultaneous trichromatic fluourescence detection of proteins. Several methods have been used to improve the detection of proteins on membranes, including glutaraldehyde treatment of nitrocellulose blots, elimination of keratin artifacts in immunoblots probed with polyclonal antibodies and the washing of immunoblots with excessive water and manipulation of Tween-20 in wash buffer. These methods are briefly reviewed in this chapter.