A blueprint of the amino acid biosynthesis network of hemiascomycetes
A blueprint of the amino acid biosynthesis network of hemiascomycetes
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DOI：
10.1111/1567-1364.12205
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发表时间：
2014-11-01
影响因子：
3.2
通讯作者：
Blank, Lars M.
中科院分区：
文献类型：
--
作者：
Foerster, Jan;Halbfeld, Christoph;Blank, Lars M.
酿酒酵母的生物合成途径的结构和调控已被广泛地详细介绍。对于其他半子囊菌，基因组序列主要是可用的，而关于它们的生化信息却很少。由此产生的用于基础科学和生物技术研究的生化网络往往受到来自酿酒酵母的数据的偏见，假设物种之间往往存在隐含的保守结构。我们研究了横跨系统发育分支的9个半子囊菌的氨基酸生物合成网络结构。遗传清单中的差异包括同工酶的存在和不同以及途径的区隔。值得注意的是，没有两个半子囊菌具有相同的基因清单。例如，在巴斯德毕赤酵母中缺乏线粒体IPMS同工酶和只存在一个拷贝的支链氨基酸氨基转移酶，这表明亮氨酸的生物合成途径是完全不同的。我们的发现表明，IPMS和BCAA氨基转移酶仅位于巴斯德毕赤酵母的胞浆中，需要纠正该物种的亮氨酸生物合成途径布局。我们的结果支持谨慎使用酿酒酵母的信息，并共同努力填补其他物种的知识空白。这样的分析将在生物技术学科中做出贡献，例如蛋白质生产和隔间工程。
The structure and regulation of biosynthesis pathways in Saccharomyces cerevisiae have been detailed extensively. For other hemiascomycetes, genomic sequences are primarily available, whereas biochemical information on them is scarce. The resulting biochemical networks that are used for research in basic science and biotechnology are often biased by data from S.cerevisiae, assuming that there are often implicitly conserved structures between species. We examined the structure of the amino acid biosynthesis network in nine hemiascomycetes, spanning the phylogenetic clade. Differences in the genetic inventory included the presence and absence of isoenzymes and compartmentation of the pathways. Notably, no two hemiascomycetes had identical genetic inventories. For example, the lack of the mitochondrial IPMS isoenzyme and presence of only one copy of the BCAA aminotransferase in Pichia pastoris indicate a disparately compartmented leucine biosynthesis pathway. Our findings suggest that IPMS and BCAA aminotransferase are solely located in the cytosol of P.pastoris, requiring correction of the leucine biosynthesis pathway layout in this species. Our results argue for careful use of information from S.cerevisiae and for joint efforts to fill the knowledge gaps in other species. Such analysis will lead to contributions in biotechnology disciplines, such as protein production and compartment engineering.