Right ventricular systolic pressure load alters myocyte maturation in fetal sheep
Right ventricular systolic pressure load alters myocyte maturation in fetal sheep
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DOI：
10.1152/ajpregu.2000.279.4.r1157
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发表时间：
2000-10-01
影响因子：
2.8
通讯作者：
Thornburg, KL
中科院分区：
文献类型：
--
作者：
Barbera, A;Giraud, GD;Thornburg, KL
研究了右心室收缩压（RVSP）负荷对胎儿心肌细胞大小和成熟的影响。在8只装载rvsp的近期胎羊中，肺动脉（PA）压从平均47.4 +/- 5.0 （+/- sd）增加到71 +/- 13.6 mmHg (P < 0.0001)，持续10天。RVSP负荷后，急性PA闭塞的右心室产生的最大压力增加：78 +/- 7 ~ 101 +/- 15 mmHg （P < 0.005）。rvsp负荷心脏（44.7 +/- 8.4 g）比5个非负荷心脏（31.8 +/- 0.2 g, P < 0.03）重；心体重量比（分别为10.9 +/- 1.1和6.5 +/- 0.9 g/kg, P < 0.0001）。RVSP-RV肌细胞比未负载的RV肌细胞长（101.3 +/- 10.2 mm） （88.2 +/- 8.1 μ m； P, 0.02），双核发生率（82 +/- 13%）比未负载的肌细胞高（63 +/- 7%；P, 0.02）。加载rvsp的心肌细胞的肌原纤维体积小于未加载的心肌细胞（44.1 +/- 4.4%和56.1 +/- 2.6%;P < 0.002）。我们得出结论，右心室收缩负荷1)导致右心室肌细胞增大，2)对左心室肌细胞大小有轻微影响，3)刺激右心室肌细胞成熟（增加右心室肌细胞双核）。心肌细胞体积数据表明右心室收缩负荷刺激增生性和肥厚性生长。
The effects of right ventricular (RV) systolic pressure (RVSP) load on fetal myocyte size and maturation were studied. Pulmonary artery (PA) pressure was increased by PA occlusion from mean 47.4 +/- 5.0 (+/-SD) to 71 +/- 13.6 mmHg (P < 0.0001) in eight RVSP-loaded near-term fetal sheep for 10 days. The maximal pressure generated by the RV with acute PA occlusion increased after RVSP load: 78 +/- 7 to 101 +/- 15 mmHg (P < 0.005). RVSP-load hearts were heavier (44.7 +/- 8.4 g) than five nonloaded hearts (31.8 +/- 0.2 g; P < 0.03); heart-to-body weight ratio (10.9 +/- 1.1 and 6.5 +/- 0.9 g/kg, respectively; P < 0.0001). RVSP-RV myocytes were longer (101.3 +/- 10.2 mm) than nonloaded RV myocytes (88.2 +/- 8.1 mu m; P, 0.02) and were more often binucleated (82 +/- 13%) than nonloaded myocytes (63 +/- 7%; P, 0.02). RVSP-loaded myocytes had less myofibrillar volume than did nonloaded hearts (44.1 +/- 4.4% and 56.1 +/- 2.6%; P < 0.002). We conclude that RV systolic load 1) leads to RV myocyte enlargement, 2) has minor effects on left ventricular myocyte size, and 3) stimulates maturation (increased RV myocyte binucleation). Myocyte volume data suggest that RV systolic loading stimulates both hyperplastic and hypertrophic growth.