LONG-LASTING CHANGES IN THE ANATOMY OF THE OLFACTORY BULB AFTER IONIZING IRRADIATION AND BONE MARROW TRANSPLANTATION
LONG-LASTING CHANGES IN THE ANATOMY OF THE OLFACTORY BULB AFTER IONIZING IRRADIATION AND BONE MARROW TRANSPLANTATION
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DOI：
10.1016/j.neuroscience.2010.10.082
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发表时间：
2011-01-26
期刊：
影响因子：
3.3
通讯作者：
Alonso, J. R.
中科院分区：
文献类型：
--
作者：
Diaz, D.;Recio, J. S.;Alonso, J. R.
成人大脑被认为是一个抗辐射器官，因为它主要由非分裂细胞组成。然而，在成年动物中，有几个神经原性脑区受到电离辐射的影响，其可塑性和恢复能力仍不清楚。在这里，小鼠用最小致死剂量的辐射照射，以确定其对脑室下区（SVZ），吻侧迁移流（RMS）和嗅球（OB）的影响。这些区域经历了细胞增殖和随后的形态学改变，伴随着专利反应性神经胶质增生显着减少。在放射治疗后还进行了骨髓干细胞（BMSC）移植，以使小鼠存活，从而分析长期影响。随着时间的推移，正常的增殖率没有恢复，尽管骨髓来源的细胞到达大脑，但它们没有被纳入SVZ-RMS-OB通路以试图挽救受损区域。由于神经发生在OB中产生新的中间神经元，因此对OB的体积和分层进行了分析。OB体积在出生后300天（P300）显著缩小，这种缩小影响到室管膜周围白色物质、颗粒细胞层、外网状层和肾小球层。这些结果应该在使用BMSC的细胞疗法中考虑，因为这些细胞到达脑，尽管它们不能恢复在神经源性区域产生的损伤。因此，这项研究为电离辐射的长期影响提供了新的见解，广泛用于动物实验，甚至用于人类的临床治疗。(C)2011年IBRO。由爱思唯尔有限公司出版。保留所有权利。
The adult brain is considered to be a radioresistant organ since it is mainly composed of non-dividing cells. However, in adult animals there are a few neurogenic brain areas that are affected by ionizing radiation whose plasticity and capacity for recovery are still unclear. Here, mice were irradiated with a minimal lethal dose of radiation in order to determine its effects on the subventricular zone (SVZ), the rostral migratory stream (RMS), and the olfactory bulb (OB). These regions underwent a dramatic reduction in cell proliferation and ensuing morphological alterations, accompanied by a patent reactive gliosis. Bone marrow stem cell (BMSC) transplants were also performed after the radiation treatment to allow the mouse survival with a view to analyzing longterm effects. Normal proliferation rates were not recovered over time and although bone marrow-derived cells reached the brain, they were not incorporated into the SVZ-RMS-OB pathway in an attempt to rescue the damaged regions. Since neurogenesis produces new interneurones in the OB, thus feeding cell turnover, the volume and lamination of the OB were analyzed. The volume of the OB proved to be dramatically reduced at postnatal day 300 (P300), and this shrinkage affected the periependymal white matter, the granule cell layer, the external plexiform layer, and the glomerular layer. These results should be taken into account in cell therapies employing BMSC, since such cells reach the encephalon, although they cannot restore the damage produced in neurogenic areas. This study thus provides new insight into the long-term effects of ionizing radiation, widely employed in animal experimentation and even in clinical therapies for human beings. (C) 2011 IBRO. Published by Elsevier Ltd. All rights reserved.